We describe a procedure for quantification of vitamin K-1 in human plasma by HPLC. Samples, enriched with a vitamin K derivative as internal standard, were deproteinized, purified on polymeric RP-SPE cartridges and injected into HPLC equipped with a post-column on-line zinc metal reactor and a fluorometric detector. Median level in blood donors (n = 87) was 1.967 nmol/L(0.93-4.01, 5th-95th percentiles), with a significant correlation between plasma levels and age (r = 0.276, p = 0.00958) and a lower (not significant) value in women than in men. This method, easy-to-handle and with a high throughput, can be used to identify covert states of vitamin K intake deficiency in patients thus at risk of alterations in blood clotting or bone mineralization.
Titolo: | Determination of vitamin K1 in plasma by solid phase extraction and HPLC with fluorescence detection |
Autori: | FAIONI, ELENA MARIA (Secondo) FONTANA, GESSICA (Penultimo) CATTANEO, MARCO NATALE (Ultimo) |
Parole Chiave: | Vitamin K-1; HPLC; Reference values; Haemostasis alteration; Bone mineralization |
Settore Scientifico Disciplinare: | Settore BIO/10 - Biochimica Settore MED/09 - Medicina Interna |
Data di pubblicazione: | 15-gen-2009 |
Rivista: | |
Tipologia: | Article (author) |
Data ahead of print / Data di stampa: | 25-dic-2008 |
Digital Object Identifier (DOI): | 10.1016/j.jchromb.2008.12.044 |
Appare nelle tipologie: | 01 - Articolo su periodico |
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