A robust method for coating polydimethylsiloxane (PDMS) allowing covalent binding of biomolecules is reported. The coating is based on adsorption of a copolymer, copoly(DMA-NAS-MAPS), bearing functional groups. The polymer is synthesized by free radical polymerization; it self-adsorbs onto the PDMS very rapidly, typically in 30 min, even without any PDMS surface pre-treatment. PDMS slides coated by copoly(DMA-NAS-MAPS) covalently bind amino-modified DNA fragments and have been tested in bacterial genotyping experiments. The usefulness of this functional coating on PDMS structures in lab-on-chip devices powered by microfluidics has been demonstrated. The copoly(DMA-NAS-MAPS) coated PDMS slides were used as flexible components of a microcell in semi-automated DNA microarray experiments for rapid food pathogen identification. © 2008 Elsevier B.V. All rights reserved.
Functionalization of poly(dimethylsiloxane) by chemisorption of copolymers: DNA microarrays for pathogen detection / M. Cretich, S. Valentina, D. Francesco, G. Di Carlo, C. Oldani, C. Marcella. - In: SENSORS AND ACTUATORS. B, CHEMICAL. - ISSN 0925-4005. - 132:1(2008), pp. 258-264.
Functionalization of poly(dimethylsiloxane) by chemisorption of copolymers: DNA microarrays for pathogen detection
G. Di Carlo;C. Oldani;
2008
Abstract
A robust method for coating polydimethylsiloxane (PDMS) allowing covalent binding of biomolecules is reported. The coating is based on adsorption of a copolymer, copoly(DMA-NAS-MAPS), bearing functional groups. The polymer is synthesized by free radical polymerization; it self-adsorbs onto the PDMS very rapidly, typically in 30 min, even without any PDMS surface pre-treatment. PDMS slides coated by copoly(DMA-NAS-MAPS) covalently bind amino-modified DNA fragments and have been tested in bacterial genotyping experiments. The usefulness of this functional coating on PDMS structures in lab-on-chip devices powered by microfluidics has been demonstrated. The copoly(DMA-NAS-MAPS) coated PDMS slides were used as flexible components of a microcell in semi-automated DNA microarray experiments for rapid food pathogen identification. © 2008 Elsevier B.V. All rights reserved.
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