For the design of new synthetic substrates for the assay of pancreatic lipases activity, acyl dialkylglycerols of variable chain length were prepared. Titrimetric assay of these substrates showed the highest lipolytic activity of porcine pancreas lipase (pPL) with butanoyl dibutylglycerol. The activity is lower but comparable to that shown by pPL towards the classical substrate tributyrin. The 4-nitrophenylcarbonate of 1,2-di-O-butylglycerol, has been prepared and proposed as synthetic substrate for a new spectrophotometric assay of pancreatic lipases.

Lipolytic activity of porcine pancreas lipase on fatty acid esters of dialkylglycerols: a structural basis for the design of new substrates for the assay of pancreatic lipases activity / P. Ciuffreda, A. Loseto, A. Manzocchi, E. Santaniello. - In: CHEMISTRY AND PHYSICS OF LIPIDS. - ISSN 0009-3084. - 111:2(2001), pp. 105-110.

Lipolytic activity of porcine pancreas lipase on fatty acid esters of dialkylglycerols: a structural basis for the design of new substrates for the assay of pancreatic lipases activity

P. Ciuffreda
Primo
;
A. Manzocchi
Penultimo
;
E. Santaniello
Ultimo
2001

Abstract

For the design of new synthetic substrates for the assay of pancreatic lipases activity, acyl dialkylglycerols of variable chain length were prepared. Titrimetric assay of these substrates showed the highest lipolytic activity of porcine pancreas lipase (pPL) with butanoyl dibutylglycerol. The activity is lower but comparable to that shown by pPL towards the classical substrate tributyrin. The 4-nitrophenylcarbonate of 1,2-di-O-butylglycerol, has been prepared and proposed as synthetic substrate for a new spectrophotometric assay of pancreatic lipases.
Dialkylglycerols; Lipolytic activity; Pancreatic lipase; Spectrophotometric assay; Titrimetric assay
Settore BIO/10 - Biochimica
2001
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/5398
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