As no studies before now have thoroughly investigated the risk associated with the exposure to low-dose ionizing radiations in patients undergoing catheter ablation (CA), we aimed to evaluate the oxidative and DNA damage in 59 CA patients (10 of whom received N-acetylcysteine (NAC) prior the procedure). A burst of oxidized/reduced glutathione ratio (GSSG/GSH) was observed 3 hours after procedure that was diminished by NAC administration. 8-hydroxy-2'-deoxyguanosine (8-OHdG) concentrations, index of oxidative DNA damage, showed a peak 24 hours after CA. A positive correlation between 8-OHdG peak and fluoroscopy time and a negative correlation among 8-OHdG decrease (from the peak to 48 hours after CA) and all procedure parameters were found. Furthermore, DNA tail percentages (which reflect the extent and the number of DNA strand breaks) positively correlated with 8-OHdG concentrations. This study evaluates for the first time the kinetic of oxidative damage in patients undergoing catheter ablation procedure. Our findings raise the question of whether 8-OHdG can be used as a circulating biomarker of DNA oxidative damage induced by low-dose ionizing radiations in different clinical settings.

Does fluoroscopy induce DNA oxidative damage in patients undergoing catheter ablation? / L. Turnu, B. Porro, V. Alfieri, A. Di Minno, E. Russo, S. Barbieri, A. Bonomi, A. Dello Russo, C. Tondo, Y. D'Alessandra, V. Cavalca, E. Tremoli, G.I. Colombo, M. Casella. - In: ANTIOXIDANTS & REDOX SIGNALING. - ISSN 1523-0864. - 28:12(2018 Apr 20), pp. 1137-1143. [10.1089/ars.2017.7334]

Does fluoroscopy induce DNA oxidative damage in patients undergoing catheter ablation?

L. Turnu;B. Porro;V. Alfieri;A. Di Minno;C. Tondo;Y. D'Alessandra;V. Cavalca;E. Tremoli;G.I. Colombo;
2018-04-20

Abstract

As no studies before now have thoroughly investigated the risk associated with the exposure to low-dose ionizing radiations in patients undergoing catheter ablation (CA), we aimed to evaluate the oxidative and DNA damage in 59 CA patients (10 of whom received N-acetylcysteine (NAC) prior the procedure). A burst of oxidized/reduced glutathione ratio (GSSG/GSH) was observed 3 hours after procedure that was diminished by NAC administration. 8-hydroxy-2'-deoxyguanosine (8-OHdG) concentrations, index of oxidative DNA damage, showed a peak 24 hours after CA. A positive correlation between 8-OHdG peak and fluoroscopy time and a negative correlation among 8-OHdG decrease (from the peak to 48 hours after CA) and all procedure parameters were found. Furthermore, DNA tail percentages (which reflect the extent and the number of DNA strand breaks) positively correlated with 8-OHdG concentrations. This study evaluates for the first time the kinetic of oxidative damage in patients undergoing catheter ablation procedure. Our findings raise the question of whether 8-OHdG can be used as a circulating biomarker of DNA oxidative damage induced by low-dose ionizing radiations in different clinical settings.
8-hydroxy-2′-deoxyguanosine; DNA damage; catheter ablation; fluoroscopy; glutathione; oxidative stress; radiological risk
Settore BIO/12 - Biochimica Clinica e Biologia Molecolare Clinica
Settore BIO/14 - Farmacologia
Settore MED/11 - Malattie dell'Apparato Cardiovascolare
27-nov-2017
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/528263
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