During growth, the oocyte accumulates mRNAs that will be required in the later stages of oogenesis and early embryogenesis until the activation of the embryonic genome. Each of these developmental stages is controlled by multiple regulatory mechanisms that ensure proper protein production. Thus mRNAs are stabilized, stored, recruited, polyadenylated, translated and/or degraded over a period of several days. As a consequence, understanding the biological significance of changes in the abundance of transcripts during oocyte growth and differentiation is rather complex. Nevertheless the availability of transcriptomic platforms applicable to scarce samples such as oocytes has generated large amounts of data that depict the transcriptome of oocytes under different conditions. Despite several technical constrains related to protein determination in oocytes that still limit the possibility to verify certain hypothesis, it is now possible to use mRNA levels to start building plausible scenarios. To start deciphering the changes in the level of specific mRNAs involved in chromatin remodelling, we have performed a meta-analysis of existing microarray datasets from germinal vesicle (GV) stage bovine oocytes during the final stages of oocyte differentiation. We then analysed the expression profiles of histone and histone-remodelling enzyme mRNAs and correlated these with the major histone modifications known to occur at the same period, based on data available in the literature. We believe that this approach could reveal the function of specific enzymes in the oocyte. In turn, this information will be useful in future studies, which final ambitious goal is to decipher the 'oocyte-specific histone code'.

Accumulation of Chromatin Remodelling Enzyme and Histone Transcripts in Bovine Oocytes / V. Lodde, A.M. Luciano, F. Franciosi, R. Labrecque, M.A. Sirard (RESULTS AND PROBLEMS IN CELL DIFFERENTIATION). - In: Oocytes / J.Z. Kubiak, M. Kloc. - [s.l] : Springer, 2017 Jan. - ISBN 9783319608549. - pp. 223-255 [10.1007/978-3-319-60855-6_11]

Accumulation of Chromatin Remodelling Enzyme and Histone Transcripts in Bovine Oocytes

V. Lodde
Primo
;
A.M. Luciano
Secondo
;
F. Franciosi;
2017

Abstract

During growth, the oocyte accumulates mRNAs that will be required in the later stages of oogenesis and early embryogenesis until the activation of the embryonic genome. Each of these developmental stages is controlled by multiple regulatory mechanisms that ensure proper protein production. Thus mRNAs are stabilized, stored, recruited, polyadenylated, translated and/or degraded over a period of several days. As a consequence, understanding the biological significance of changes in the abundance of transcripts during oocyte growth and differentiation is rather complex. Nevertheless the availability of transcriptomic platforms applicable to scarce samples such as oocytes has generated large amounts of data that depict the transcriptome of oocytes under different conditions. Despite several technical constrains related to protein determination in oocytes that still limit the possibility to verify certain hypothesis, it is now possible to use mRNA levels to start building plausible scenarios. To start deciphering the changes in the level of specific mRNAs involved in chromatin remodelling, we have performed a meta-analysis of existing microarray datasets from germinal vesicle (GV) stage bovine oocytes during the final stages of oocyte differentiation. We then analysed the expression profiles of histone and histone-remodelling enzyme mRNAs and correlated these with the major histone modifications known to occur at the same period, based on data available in the literature. We believe that this approach could reveal the function of specific enzymes in the oocyte. In turn, this information will be useful in future studies, which final ambitious goal is to decipher the 'oocyte-specific histone code'.
Bovine; Chromatin configuration; Epigenetic modification; Germinal vesicle; Histone; Oocyte; Transcriptome; mRNA
Settore VET/01 - Anatomia degli Animali Domestici
Settore BIO/06 - Anatomia Comparata e Citologia
Settore VET/02 - Fisiologia Veterinaria
Settore BIO/11 - Biologia Molecolare
Settore VET/10 - Clinica Ostetrica e Ginecologia Veterinaria
gen-2017
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/526909
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