AGMA1, a prevailingly cationic, guanidine-bearing, linear, amphoteric polyamidoamine is an effective siRNA condensing agent. Here two AGMA1 samples of different molecular weight, i.e. AGMA1-5 and AGMA1-10 were evaluated as siRNA condensing agents and transfection promoters. AGMA1-10 formed stable polyplexes with a size lower than 50 nm and positive zeta potential. AGMA1-5 polyplexes were larger, about 100 nm in size. AGMA1â10 polyplexes, but not AGMA1-5 proved to be an effective intracellular siRNA carrier, able to trigger gene silencing in Hela and PC3 cell lines without eliciting cytotoxic effects. AGMA1-10 knocked down AKT-1 expression upon transfection with an AKT-1 specific siRNA. The polyplex entry mechanism was investigated and was mediated by macropinocytosis. In conclusion, AGMA1 has potential as an efficient, non-toxic tool for the intracellular delivery of siRNA and warrants further investigation.
The AGMA1 polyamidoamine mediates the efficient delivery of siRNA / R. Cavalli, L. Primo, R. Sessa, G. Chiaverina, L. Di Blasio, J. Alongi, A.G. Manfredi, E. Ranucci, P. Ferruti. - In: JOURNAL OF DRUG TARGETING. - ISSN 1061-186X. - 25:9-10(2017 Aug 18), pp. 891-898.
|Titolo:||The AGMA1 polyamidoamine mediates the efficient delivery of siRNA|
RANUCCI, ELISABETTA (Penultimo)
FERRUTI, PAOLO (Ultimo)
|Parole Chiave:||Polyamidoamine; AGMA1; siRNA; AGMA1/siRNA polyplexes; intracellular siRNA delivery; gene silencing|
|Settore Scientifico Disciplinare:||Settore CHIM/04 - Chimica Industriale|
|Data di pubblicazione:||18-ago-2017|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1080/1061186X.2017.1363215|
|Appare nelle tipologie:||01 - Articolo su periodico|