Aim: We investigate MAdCAM-1 as a reliable target to detect active bowel inflammation for selective noninvasive nanodiagnostics. Materials & methods: We coupled anti-MAdCAM-1 antibodies to manganese oxide nanoparticles, and analyzed nanoconjugate biodistribution and safety in murine model of inflammatory bowel disease by imaging and histology. Results: Nanoparticles were stable and nontoxic. Upon administration in colitic mice, anti-MAdCAM-1 functionalized nanoparticles preferentially localized in the inflamed bowel, whereas untargeted nanoparticles were more rapidly washed out. Nanoparticles did not induce lesions in nontarget organs. Conclusion: Anti-MAdCAM-1 functionalized nanoparticles detected active bowel inflammation foci, accurately following MAdCAM-1 expression pattern. These nanoconjugates could be a promising noninvasive imaging system for an early and accurate follow-up in patients affected by acute colitis.
Nano-targeting of mucosal addressin cell adhesion molecule-1 identifies bowel inflammation foci in murine model / M. Truffi, M. Colombo, J. Penaranda Avila, L. Sorrentino, F. Colombo, M. Monieri, V. Collico, P. Zerbi, E. Longhi, R. Allevi, D. Prosperi, F. Corsi. - In: NANOMEDICINE. - ISSN 1743-5889. - 12:13(2017 Jul), pp. 1547-1560.
Nano-targeting of mucosal addressin cell adhesion molecule-1 identifies bowel inflammation foci in murine model
M. TruffiPrimo
;L. Sorrentino;F. Colombo;M. Monieri;P. Zerbi;E. Longhi;R. Allevi;F. Corsi
Ultimo
2017
Abstract
Aim: We investigate MAdCAM-1 as a reliable target to detect active bowel inflammation for selective noninvasive nanodiagnostics. Materials & methods: We coupled anti-MAdCAM-1 antibodies to manganese oxide nanoparticles, and analyzed nanoconjugate biodistribution and safety in murine model of inflammatory bowel disease by imaging and histology. Results: Nanoparticles were stable and nontoxic. Upon administration in colitic mice, anti-MAdCAM-1 functionalized nanoparticles preferentially localized in the inflamed bowel, whereas untargeted nanoparticles were more rapidly washed out. Nanoparticles did not induce lesions in nontarget organs. Conclusion: Anti-MAdCAM-1 functionalized nanoparticles detected active bowel inflammation foci, accurately following MAdCAM-1 expression pattern. These nanoconjugates could be a promising noninvasive imaging system for an early and accurate follow-up in patients affected by acute colitis.File | Dimensione | Formato | |
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