1. Allogamus auricollis cell-free hemolymph proteins were analyzed by SDS-PAGE. Under reducing conditions the gel pattern showed two main components (83 and 76 kDa) and some lesser bands. 2. After native PAGE, a single band showed phenoloxidase activity by in situ enzymatic staining. 3. Spectrophotometric analysis of the cell-free plasma fraction was carried out with substrate and PTU as inhibitor.
Biochemical evidence of phenoloxidase activity (pro-PO system) in larvae of Allogamus auricollis (Insecta, Trichoptera) / M.F. Brivio, M. Pagani, G. Scarì. - In: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY. B, COMPARATIVE BIOCHEMISTRY. - ISSN 0305-0491. - 102:4(1992), pp. 867-871. [10.1016/0305-0491(92)90094-8]
Biochemical evidence of phenoloxidase activity (pro-PO system) in larvae of Allogamus auricollis (Insecta, Trichoptera)
G. ScarìUltimo
1992
Abstract
1. Allogamus auricollis cell-free hemolymph proteins were analyzed by SDS-PAGE. Under reducing conditions the gel pattern showed two main components (83 and 76 kDa) and some lesser bands. 2. After native PAGE, a single band showed phenoloxidase activity by in situ enzymatic staining. 3. Spectrophotometric analysis of the cell-free plasma fraction was carried out with substrate and PTU as inhibitor.
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