Transplantation of pancreatic islets or stem cell derived insulin secreting cells is an attractive treatment strategy for diabetes. However, islet transplantation is associated with several challenges including function-loss associated with dispersion and limited vascularization as well as the need for continuous immunosuppression. To overcome these limitations, here we present a novel 3D printed and functionalized encapsulation system for subcutaneous engraftment of islets or islet like cells. The devices were 3D printed with polylactic acid and the surfaces treated and patterned to increase the hydrophilicity, cell attachment, and proliferation. Surface treated encapsulation systems were implanted with growth factor enriched platelet gel, which helped to create a vascularized environment before loading human islets. The device protected the encapsulated islets from acute hypoxia and kept them functional. The adaptability of the encapsulation system was demonstrated by refilling some of the experimental groups transcutaneously with additional islets.

3D Printed Vascularized Device for Subcutaneous Transplantation of Human Islets / M. Farina, A. Ballerini, D.W. Fraga, E. Nicolov, M. Hogan, D. Demarchi, F. Scaglione, O.M. Sabek, P. Horner, U. Thekkedath, O.A. Gaber, A. Grattoni. - In: BIOTECHNOLOGY JOURNAL. - ISSN 1860-6768. - 12:9(2017 Sep), pp. 1700169.1-1700169.5. [10.1002/biot.201700169]

3D Printed Vascularized Device for Subcutaneous Transplantation of Human Islets

M. Farina
Primo
;
A. Ballerini
Secondo
;
F. Scaglione;
2017

Abstract

Transplantation of pancreatic islets or stem cell derived insulin secreting cells is an attractive treatment strategy for diabetes. However, islet transplantation is associated with several challenges including function-loss associated with dispersion and limited vascularization as well as the need for continuous immunosuppression. To overcome these limitations, here we present a novel 3D printed and functionalized encapsulation system for subcutaneous engraftment of islets or islet like cells. The devices were 3D printed with polylactic acid and the surfaces treated and patterned to increase the hydrophilicity, cell attachment, and proliferation. Surface treated encapsulation systems were implanted with growth factor enriched platelet gel, which helped to create a vascularized environment before loading human islets. The device protected the encapsulated islets from acute hypoxia and kept them functional. The adaptability of the encapsulation system was demonstrated by refilling some of the experimental groups transcutaneously with additional islets.
3D printing; diabetes; encapsulations; islets; transplantations
Settore BIO/14 - Farmacologia
set-2017
Article (author)
File in questo prodotto:
File Dimensione Formato  
Farina_et_al-2017-Biotechnology_Journal.pdf

accesso riservato

Descrizione: online first
Tipologia: Publisher's version/PDF
Dimensione 1.86 MB
Formato Adobe PDF
1.86 MB Adobe PDF   Visualizza/Apri   Richiedi una copia
Farina_et_al-2017-Biotechnology_Journal.pdf

accesso riservato

Tipologia: Publisher's version/PDF
Dimensione 1.86 MB
Formato Adobe PDF
1.86 MB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/522541
Citazioni
  • ???jsp.display-item.citation.pmc??? 23
  • Scopus 68
  • ???jsp.display-item.citation.isi??? 65
social impact