Purpose of Review Spurred by the successful application of structural vaccinology to other challenging bacterial and viral pathogens, we review the possibility of exploiting 3D structure computational-based recombinant antigen engineering strategies for the development of a protective melioidosis vaccine. Recent Findings Structure-based epitope design approaches in the melioidosis field are preliminary and applied essentially by one research network. By combining Burkholderia pseudomallei antigen 3D structures and in silico epitope discovery methods, a panel of synthetic epitope peptides were designed and tested for their B and T cell stimulatory activities. Several peptides were found to be serodiagnostic for B. pseudomallei infection and two elicited bactericidal antibodies. Summary A significant amount of B. pseudomallei antigen structures, epitopes, and immunological data is available. Future challenges will be to test all available B. pseudomallei epitopes, focusing on combing multiple B/T cell epitopes onto a single scaffold to generate components, stimulating both arms of the immune system.
Structural vaccinology for melioidosis vaccine design and immunodiagnostics / A. Gori, M. Bolognesi, G. Colombo, L.J. Gourlay. - In: CURRENT TROPICAL MEDICINE REPORTS. - ISSN 2196-3045. - 4:3(2017 Sep 01), pp. 103-110. [10.1007/s40475-017-0117-3]
Structural vaccinology for melioidosis vaccine design and immunodiagnostics
A. GoriPrimo
;M. BolognesiSecondo
;L.J. GourlayUltimo
2017
Abstract
Purpose of Review Spurred by the successful application of structural vaccinology to other challenging bacterial and viral pathogens, we review the possibility of exploiting 3D structure computational-based recombinant antigen engineering strategies for the development of a protective melioidosis vaccine. Recent Findings Structure-based epitope design approaches in the melioidosis field are preliminary and applied essentially by one research network. By combining Burkholderia pseudomallei antigen 3D structures and in silico epitope discovery methods, a panel of synthetic epitope peptides were designed and tested for their B and T cell stimulatory activities. Several peptides were found to be serodiagnostic for B. pseudomallei infection and two elicited bactericidal antibodies. Summary A significant amount of B. pseudomallei antigen structures, epitopes, and immunological data is available. Future challenges will be to test all available B. pseudomallei epitopes, focusing on combing multiple B/T cell epitopes onto a single scaffold to generate components, stimulating both arms of the immune system.File | Dimensione | Formato | |
---|---|---|---|
Proofs_LG.pdf
accesso aperto
Tipologia:
Post-print, accepted manuscript ecc. (versione accettata dall'editore)
Dimensione
1.31 MB
Formato
Adobe PDF
|
1.31 MB | Adobe PDF | Visualizza/Apri |
Pubblicazioni consigliate
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.