We investigated the presence of enzymes on the surface of Drosophila melanogaster spermatozoa that might bind to the carbohydrate residues of the egg shell. Spectrophotometric and fluorimetric studies were used on whole spermatozoa to assay galactosyltransferase and glycosidase activities. No galaclosyltransferase is present on the sperm surface, whereas two glycosidases, beta-N-acetylglucosaminidase (GlcNAc'ase) and alpha-mannosidase (Man'ase), have been evidenced. They have an optimal pH of 6-6.5 and 4, respectively. The same glycosidases were detected as soluble forms probably secreted by the seminal vesicle epithelium. We suggest that these enzymes might be involved in the recognition of alpha-mannose and beta-N-acetylglucosamine residues present on the egg shell at the site of sperm entry.
Glycosidases are present on the surface of Drosophila melanogaster spermatozoa / F. Cattaneo, M.E Pasini, M.E. Perotti,. - In: MOLECULAR REPRODUCTION AND DEVELOPMENT. - ISSN 1040-452X. - 48:2(1997), pp. 276-281.
|Titolo:||Glycosidases are present on the surface of Drosophila melanogaster spermatozoa|
CATTANEO, FABIO VITTORIO (Primo)
PASINI, MARIA ENRICA (Secondo)
PEROTTI, MARIA ELISA (Ultimo)
|Parole Chiave:||Fertilization; Gametes; Insects|
|Settore Scientifico Disciplinare:||Settore BIO/06 - Anatomia Comparata e Citologia|
|Data di pubblicazione:||1997|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1002/(SICI)1098-2795(199710)48:2<276::AID-MRD16>3.0.CO;2-W|
|Appare nelle tipologie:||01 - Articolo su periodico|