The present study stems from previous observations demonstrating that in the neuroblastoma cell line SK-ER3 the mRNA content of the pro-apoptotic gene Nip2 is decreased following treatment with estradiol. We investigate the content of Nip2 mRNA during the maturation of rat embryo brain and we show that Nip2 mRNA is very low at embryo day 15 and steadily increases up to day 20. At day 21 Nip2 mRNA is decreased almost to the low levels observed in the mature brain. Studies in neurons from rat embryo at day 18 show that Nip2 mRNA content is significantly decreased by exposure to estradiol at 1 nM concentration demonstrating that the observations previously done in the SK-ER3 neuroblastoma cell line can be reproduced in neurons in culture. The finding that estrogens may modulate the activity of Nip2 gene activity may be of relevance not only with regard to the effects of estradiol on brain maturation, but also for the understanding of the neuroprotective effects recently described for this hormone.
Expression of the estrogen-regulated gene Nip2 during rat brain maturation / A. Brusadelli, H. Sialino, T. Piepoli, G. Pollio, A. Maggi. - In: INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE. - ISSN 0736-5748. - 18:2-3(2000 Jun), pp. 317-320.
|Titolo:||Expression of the estrogen-regulated gene Nip2 during rat brain maturation|
BRUSADELLI, ALESSIA (Primo)
POLLIO, GIUSEPPE (Penultimo)
MAGGI, ADRIANA CATERINA (Ultimo)
|Parole Chiave:||animals; apoptosis; blotting, northern; brain; brain chemistry; calcium-binding proteins; cell differentiation; cells, cultured; estradiol; female; gene expression regulation, developmental; neurons; pregnancy; proto-oncogene proteins C-BCL-2; rna, messenger; rats; rats, sprague-dawley; carrier proteins|
|Settore Scientifico Disciplinare:||Settore BIO/09 - Fisiologia|
Settore BIO/10 - Biochimica
Settore BIO/14 - Farmacologia
|Data di pubblicazione:||giu-2000|
|Centro di ricerca:||Centro Interdipartimentale di Eccellenza per le Malattie Neurodegenerative CEND|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/S0736-5748(99)00100-8|
|Appare nelle tipologie:||01 - Articolo su periodico|