The preparation and investigation of model membranes is deserving growing interest both for the physics of complex systems, and for biology. The need of simplified models should preserve mimicking the qualifying characteristics of biological membranes, and keep non-invasive and detailed description. As a main feature, biological membranes are non-homogeneous in the disposition of components, both in the lateral and in the transverse direction. We prepared asymmetric supported membranes containing GM1 ganglioside in biomimetic proportion according to different protocols. Then, we studied their internal structure by neutron reflectometry, providing few-Angstrom sensitivity in the cross direction meanwhile avoiding radiation damage. This technique can also be profitably applied to study interactions at the membrane surface. The best protocol has proven to be the Langmuir-Blodgett/Langmuir-Schaefer depositions. Notably, also the simpler and most accessible protocol of vesicle fusion was found to be suitable for straightforward and good quality deposition of compositionally asymmetric membranes.

Building a biomimetic membrane for neutron reflectivity investigation : complexity, asymmetry and contrast / V. Rondelli, P. Brocca, N. Tranquilli, G. Fragneto, E. Del Favero, L. Cantù. - In: BIOPHYSICAL CHEMISTRY. - ISSN 0301-4622. - 229(2017 Apr 29), pp. 135-141. ((Intervento presentato al 23. convegno National Congress of the Italian Society for Pure and Applied Biophysics : Congresso nazionale della Società italiana di biofisica pura e applicata (SIBPA) tenutosi a Cortona nel 2016 [10.1016/j.bpc.2017.04.011].

Building a biomimetic membrane for neutron reflectivity investigation : complexity, asymmetry and contrast

V. Rondelli;P. Brocca;E. Del Favero;L. Cantù
2017-04-29

Abstract

The preparation and investigation of model membranes is deserving growing interest both for the physics of complex systems, and for biology. The need of simplified models should preserve mimicking the qualifying characteristics of biological membranes, and keep non-invasive and detailed description. As a main feature, biological membranes are non-homogeneous in the disposition of components, both in the lateral and in the transverse direction. We prepared asymmetric supported membranes containing GM1 ganglioside in biomimetic proportion according to different protocols. Then, we studied their internal structure by neutron reflectometry, providing few-Angstrom sensitivity in the cross direction meanwhile avoiding radiation damage. This technique can also be profitably applied to study interactions at the membrane surface. The best protocol has proven to be the Langmuir-Blodgett/Langmuir-Schaefer depositions. Notably, also the simpler and most accessible protocol of vesicle fusion was found to be suitable for straightforward and good quality deposition of compositionally asymmetric membranes.
GM1 ganglioside; neutron reflectometry; single model membrane; biomembrane asymmetry; GEMs; vesicle fusion
Settore FIS/07 - Fisica Applicata(Beni Culturali, Ambientali, Biol.e Medicin)
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/502748
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