A bimodular genetic fusion comprising a delivery module (scFv) and a capture module (SNAP) is proposed as a novel strategy for the biologically mediated site-specific covalent conjugation of targeting proteins to nanoparticles. ScFv800E6, an scFv mutant selective for HER2 antigen overexpressed in breast cancer cells was chosen as targeting ligand. The fusion protein SNAP-scFv was irreversibly immobilized on magnetofluorescent nanoparticles through the recognition between SNAP module and pegylated O 6-alkylguanine derivative. The targeting efficiency of the resulting nanoparticle against HER2-positive breast cancer cells was assessed by flow cytometry and immunofluorescence.

O6-alkylguanine-DNA transferase (SNAP) as capture module for site-specific covalent bioconjugation of targeting protein on nanoparticles / S. Mazzucchelli, M. Colombo, E. Galbiati, F. Corsi, J.M. Montenegro, W.J. Parak, D. Prosperi. - In: PROGRESS IN BIOMEDICAL OPTICS AND IMAGING. - ISSN 1605-7422. - 8595 [Proceedings of SPIE](2013), pp. 859502.1-859502.9. ((Intervento presentato al 8. convegno Colloidal nanocrystals for biomedical applications tenutosi a San Francisco (CA) nel 2013 [10.1117/12.2001648].

O6-alkylguanine-DNA transferase (SNAP) as capture module for site-specific covalent bioconjugation of targeting protein on nanoparticles

S. Mazzucchelli
;
F. Corsi;
2013

Abstract

A bimodular genetic fusion comprising a delivery module (scFv) and a capture module (SNAP) is proposed as a novel strategy for the biologically mediated site-specific covalent conjugation of targeting proteins to nanoparticles. ScFv800E6, an scFv mutant selective for HER2 antigen overexpressed in breast cancer cells was chosen as targeting ligand. The fusion protein SNAP-scFv was irreversibly immobilized on magnetofluorescent nanoparticles through the recognition between SNAP module and pegylated O 6-alkylguanine derivative. The targeting efficiency of the resulting nanoparticle against HER2-positive breast cancer cells was assessed by flow cytometry and immunofluorescence.
bioconjugation; breast cancer; capture module; fusion protein; magnetic nanoparticles; scFv; targeting module; tumor targeting; atomic and molecular physics, and optics; electronic, optical and magnetic materials; biomaterials; radiology, nuclear medicine and imaging
Settore BIO/10 - Biochimica
Settore BIO/13 - Biologia Applicata
Settore MED/06 - Oncologia Medica
Settore BIO/12 - Biochimica Clinica e Biologia Molecolare Clinica
The Society of Photo-Optical Instrumentation Engineers
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/502694
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