Previous studies suggest that PGRMC1 plays essential roles dur- ing bovine oocyte meiosis since it localizes to the centromeres at metaphase-I and II and concentrates between the separating chromosomes at ana/telophase-I. To confirm this hypothesis, we conducted a series of functional studies in which PGRMC1 activity was disturbed by using several experimental approaches and the effect on meiosis-I was assessed. In a first set of experiments, treat- ment with the PGRMC1 inhibitor (AG205) significantly affected oocyte meiosis in a dose dependent manner by: (1) decreasing the % of oocytes that extruded the first polar body (PB-I); (2) affecting meiotic progression by decreasing the % of oocytes that reached MII-stage and increasing the % of oocytes showing aberration of chromosome segregation that often resulted in the formation of scattered DNA within the cytoplasm. In a second set of experiments, a siRNA-mediated gene silencing approach was used to reduce oocytes PGRMC1 expression. Overall, PGRMC1 down-regulation mirrored AG205 effects, by (1) significantly reducing the % of oocytes extruding PB-I; (2) increasing the % of oocytes showing aberrant meiotic figures and DNA scattering. Finally in a third set of experiments, injection of an antibody to PGRMC1 had a more pro- nounced effect. In fact, this treatment significantly impaired the formation of MI plates and completion of meiosis-I. The present findings are consistent with PGRMC1 localization and with a puta- tive role in both chromosomes separation and cytokinesis.
Functional assessment of Progesterone Receptor Membrane Component 1 (PGRMC1) activity during bovine oocyte meiosis / V. Lodde, I. Tessaro, F. Franciosi, F. Raucci, L. Terzaghi, G. Sivelli, J.J. Peluso, S. Modina, A.M. Luciano. - In: ANNALS OF ANATOMY. - ISSN 0940-9602. - 207(2016 Sep), pp. 121-121. ((Intervento presentato al 10. convegno Annual Meeting of the Italian Association of the Veterinary Morphologists (AMV) tenutosi a Rome (Italy) nel 2015.
|Titolo:||Functional assessment of Progesterone Receptor Membrane Component 1 (PGRMC1) activity during bovine oocyte meiosis|
LODDE, VALENTINA (Primo)
TESSARO, IRENE (Secondo)
MODINA, SILVIA CLOTILDE (Penultimo)
LUCIANO, ALBERTO MARIA (Ultimo)
|Settore Scientifico Disciplinare:||Settore VET/01 - Anatomia degli Animali Domestici|
|Data di pubblicazione:||set-2016|
|Enti collegati al convegno:||Italian Association of the Veterinary Morphologists (AMV)|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.aanat.2016.04.012|
|Appare nelle tipologie:||01 - Articolo su periodico|