Rapid detection and quantification of molecular and microbiological contaminants in water samples represent common needs of different application fields. In this work we report a novel method enabling the multiplex optical detection of both molecular and micron-sized particle binding on a functionalized glass slide embedded in a microfluidic cell, without using fluorochrome markers nor colorimetric reaction. We characterize the self-assembly of the array of probe proteins via DNA-directed immobilization and demonstrate the detection of micro-particles on the same surface. The proposed method provides an important tool to develop novel arrays for rapid screening of bacteria.

Label-free optical detection of molecular and cellular binding on reflectionless glass / S. Biffi, G. Tagliabue, M. Travagnin, F. Damin, M. Chiari, S. Deegan, M. Tijero, J. Saez, F. Benito-Lopez, M. Salina, M. Buscaglia - In: Detailed characterization of plasmids carrying resistance genes using optical DNA mapping[s.l] : Chemical and Biological Microsystems Society, 2016. - ISBN 9780979806490. - pp. 39-40 (( Intervento presentato al 20. convegno International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS tenutosi a Dublin nel 2016.

Label-free optical detection of molecular and cellular binding on reflectionless glass

S. Biffi;TAGLIABUE, GIOVANNI;TRAVAGNIN, MARTINO;SALINA, MATTEO;M. Buscaglia
2016

Abstract

Rapid detection and quantification of molecular and microbiological contaminants in water samples represent common needs of different application fields. In this work we report a novel method enabling the multiplex optical detection of both molecular and micron-sized particle binding on a functionalized glass slide embedded in a microfluidic cell, without using fluorochrome markers nor colorimetric reaction. We characterize the self-assembly of the array of probe proteins via DNA-directed immobilization and demonstrate the detection of micro-particles on the same surface. The proposed method provides an important tool to develop novel arrays for rapid screening of bacteria.
Antibody; Bacteria detection; DNA-directed immobilization; Label-free optical detection; Lectin; Microarray; Microfluidic cell; Molecular binding; Reflective phantom interface
Settore FIS/07 - Fisica Applicata(Beni Culturali, Ambientali, Biol.e Medicin)
Next Generation Biomimetic Analytical Platforms. Building Blocks for Ubiquitous Environmental Sensor Networks
The Chemical and Biological Microsystems Society (CBMS)
Book Part (author)
File in questo prodotto:
File Dimensione Formato  
MicroTAS2016_Buscaglia.pdf

non disponibili

369.78 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/502422
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 0
  • ???jsp.display-item.citation.isi??? ND
social impact