To investigate the ability of the proteases, subtilisin and alpha-chymotrypsin (aCT), to inhibit the adhesion of Candida albicans biofilm to a polypropylene surface. The proteases were immobilized on plasma-treated polypropylene by covalently linking them with either glutaraldehyde (GA) or N'-diisopropylcarbodiimide (DIC) and N-hydroxysuccinimide (NHS). The immobilization did not negatively affect the enzyme activity and in the case of subtilisin, the activity was up to 640% higher than that of the free enzyme when using N-acetyl phenylalanine ethyl ester as the substrate. The efficacies against biofilm dispersal for the GA-linked SubC and aCT coatings were 41 and 55% higher than the control (polypropylene coated with only GA), respectively, whereas no effect was observed with enzymes immobilized with DIC and NHS. The higher dispersion efficacy observed for the proteases immobilized with GA could be both steric (proper orientation of the active site) and dynamic (higher protein mobility/flexibility). Proteases immobilized on a polypropylene surface reduced the adhesion of C. albicans biofilms and therefore may be useful in developing anti-biofilm surfaces based on non-toxic molecules and sustainable strategies.

Coating polypropylene surfaces with protease weakens the adhesion and increases the dispersion of Candida albicans cells / E. Spadoni Andreani, F. Villa, F. Cappitelli, A. Krasowska, P. Biniarz, M. Lukaszewicz, F. Secundo. - In: BIOTECHNOLOGY LETTERS. - ISSN 0141-5492. - 39:3(2017), pp. 423-428. [10.1007/s10529-016-2262-5]

Coating polypropylene surfaces with protease weakens the adhesion and increases the dispersion of Candida albicans cells

F. Villa
Secondo
;
F. Cappitelli;
2017

Abstract

To investigate the ability of the proteases, subtilisin and alpha-chymotrypsin (aCT), to inhibit the adhesion of Candida albicans biofilm to a polypropylene surface. The proteases were immobilized on plasma-treated polypropylene by covalently linking them with either glutaraldehyde (GA) or N'-diisopropylcarbodiimide (DIC) and N-hydroxysuccinimide (NHS). The immobilization did not negatively affect the enzyme activity and in the case of subtilisin, the activity was up to 640% higher than that of the free enzyme when using N-acetyl phenylalanine ethyl ester as the substrate. The efficacies against biofilm dispersal for the GA-linked SubC and aCT coatings were 41 and 55% higher than the control (polypropylene coated with only GA), respectively, whereas no effect was observed with enzymes immobilized with DIC and NHS. The higher dispersion efficacy observed for the proteases immobilized with GA could be both steric (proper orientation of the active site) and dynamic (higher protein mobility/flexibility). Proteases immobilized on a polypropylene surface reduced the adhesion of C. albicans biofilms and therefore may be useful in developing anti-biofilm surfaces based on non-toxic molecules and sustainable strategies.
antibiofilm; biofilms; candida albicans; α-chymotrpsin; proteinase; polypropylene; subtilisin
Settore AGR/16 - Microbiologia Agraria
2017
Article (author)
File in questo prodotto:
File Dimensione Formato  
10.1007_s10529-016-2262-5.pdf

accesso riservato

Tipologia: Publisher's version/PDF
Dimensione 437.25 kB
Formato Adobe PDF
437.25 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/502276
Citazioni
  • ???jsp.display-item.citation.pmc??? 5
  • Scopus 17
  • ???jsp.display-item.citation.isi??? 13
social impact