Beyond the physiology of reproduction, estrogen controls the homeostasis of several tissues. Although macrophages play a key role in tissue remodeling, the interplay with estrogen is still ill defined. Using a transcriptomic approach we first obtained a comprehensive list of genes that are differentially expressed in peritoneal macrophages in response to physiological levels of 17β-estradiol (E 2) injected in intact female mice. Our data also showed the dynamic nature of the macrophage response to E 2 and pointed to specific biological programs induced by the hormone, with cell proliferation, immune response and wound healing being the most prominent functional categories. Indeed, the exogenous administration of E 2 and, more importantly, the endogenous hormonal surge proved to support macrophage proliferation in vivo, as shown by cell cycle gene expression, BrdU incorporation and cell number. Furthermore, E 2 promoted an anti-inflammatory and pro-resolving macrophage phenotype, which converged on the induction of genes related to macrophage alternative activation and on IL-10 expression in vivo. Hormone action was maintained in an experimental model of peritoneal inflammation based on zymosan injection. These findings highlight a direct effect of estrogen on macrophage expansion and phenotypic adaptation in homeostatic conditions and suggest a role for this interplay in inflammatory pathologies.
Self-renewal and phenotypic conversion are the main physiological responses of macrophages to the endogenous estrogen surge / G. Pepe, D. Braga, T.A. Renzi, A. Villa, C. Bolego, F. D'Avila, C. Barlassina, A. Maggi, M. Locati, E. Vegeto. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 7(2017 Mar), pp. 44270.1-44270.14.
|Titolo:||Self-renewal and phenotypic conversion are the main physiological responses of macrophages to the endogenous estrogen surge|
PEPE, GIOVANNA LUISA (Primo)
BRAGA, DANIELE (Secondo)
LOCATI, MASSIMO (Penultimo)
VEGETO, ELISABETTA (Corresponding)
|Settore Scientifico Disciplinare:||Settore BIO/14 - Farmacologia|
|Progetto:||Imaging of Neuroinflammation in Neurodegenerative Diseases|
|Data di pubblicazione:||mar-2017|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1038/srep44270|
|Appare nelle tipologie:||01 - Articolo su periodico|