Beyond the physiology of reproduction, estrogen controls the homeostasis of several tissues. Although macrophages play a key role in tissue remodeling, the interplay with estrogen is still ill defined. Using a transcriptomic approach we first obtained a comprehensive list of genes that are differentially expressed in peritoneal macrophages in response to physiological levels of 17β-estradiol (E 2) injected in intact female mice. Our data also showed the dynamic nature of the macrophage response to E 2 and pointed to specific biological programs induced by the hormone, with cell proliferation, immune response and wound healing being the most prominent functional categories. Indeed, the exogenous administration of E 2 and, more importantly, the endogenous hormonal surge proved to support macrophage proliferation in vivo, as shown by cell cycle gene expression, BrdU incorporation and cell number. Furthermore, E 2 promoted an anti-inflammatory and pro-resolving macrophage phenotype, which converged on the induction of genes related to macrophage alternative activation and on IL-10 expression in vivo. Hormone action was maintained in an experimental model of peritoneal inflammation based on zymosan injection. These findings highlight a direct effect of estrogen on macrophage expansion and phenotypic adaptation in homeostatic conditions and suggest a role for this interplay in inflammatory pathologies.

Self-renewal and phenotypic conversion are the main physiological responses of macrophages to the endogenous estrogen surge / G. Pepe, D. Braga, T.A. Renzi, A. Villa, C. Bolego, F. D'Avila, C. Barlassina, A. Maggi, M. Locati, E. Vegeto. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 7(2017 Mar), pp. 44270.1-44270.14. [10.1038/srep44270]

Self-renewal and phenotypic conversion are the main physiological responses of macrophages to the endogenous estrogen surge

G. Pepe
Primo
;
D. Braga
Secondo
;
T.A. Renzi;A. Villa;F. D'Avila;C. Barlassina;A. Maggi;M. Locati
Penultimo
;
E. Vegeto
2017-03

Abstract

Beyond the physiology of reproduction, estrogen controls the homeostasis of several tissues. Although macrophages play a key role in tissue remodeling, the interplay with estrogen is still ill defined. Using a transcriptomic approach we first obtained a comprehensive list of genes that are differentially expressed in peritoneal macrophages in response to physiological levels of 17β-estradiol (E 2) injected in intact female mice. Our data also showed the dynamic nature of the macrophage response to E 2 and pointed to specific biological programs induced by the hormone, with cell proliferation, immune response and wound healing being the most prominent functional categories. Indeed, the exogenous administration of E 2 and, more importantly, the endogenous hormonal surge proved to support macrophage proliferation in vivo, as shown by cell cycle gene expression, BrdU incorporation and cell number. Furthermore, E 2 promoted an anti-inflammatory and pro-resolving macrophage phenotype, which converged on the induction of genes related to macrophage alternative activation and on IL-10 expression in vivo. Hormone action was maintained in an experimental model of peritoneal inflammation based on zymosan injection. These findings highlight a direct effect of estrogen on macrophage expansion and phenotypic adaptation in homeostatic conditions and suggest a role for this interplay in inflammatory pathologies.
Multidisciplinary
Settore BIO/14 - Farmacologia
Imaging of Neuroinflammation in Neurodegenerative Diseases
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/501751
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