Eukaryotic initiation factor 6 (eIF6, alias p27BBP) is required for the biogenesis of 60S ribosomal subunits. eIF6 expression levels are tightly regulated in vivo, where they correlate with cellular growth. We analyzed how transcriptional regulation of eIF6 is achieved. We show that the human eIF6 promoter contains consensus sites for the GABP (GA-binding protein) transcription factor complex. Functional analysis of GABP consensus sequences by point mutations, EMSA (electrophoretic mobility shift assay) and a dominant negative mutant indicates that GABP is essential for eIF6 promoter activity. These data strengthen the hypothesis that GABP is a global regulator of ribosome synthesis.

GABP complex regulates transcription of eIF6 (p27BBP), an essential trans-acting factor in ribosome biogenesis / A. Donadini, F. Giacopelli, R. Ravazzolo, V. Gandin, P.C. Marchisio, S. Biffo. - In: FEBS LETTERS. - ISSN 0014-5793. - 580:8(2006), pp. 1983-1987.

GABP complex regulates transcription of eIF6 (p27BBP), an essential trans-acting factor in ribosome biogenesis

V. Gandin;S. Biffo
2006

Abstract

Eukaryotic initiation factor 6 (eIF6, alias p27BBP) is required for the biogenesis of 60S ribosomal subunits. eIF6 expression levels are tightly regulated in vivo, where they correlate with cellular growth. We analyzed how transcriptional regulation of eIF6 is achieved. We show that the human eIF6 promoter contains consensus sites for the GABP (GA-binding protein) transcription factor complex. Functional analysis of GABP consensus sequences by point mutations, EMSA (electrophoretic mobility shift assay) and a dominant negative mutant indicates that GABP is essential for eIF6 promoter activity. These data strengthen the hypothesis that GABP is a global regulator of ribosome synthesis.
Beta4 binding protein (p27BBP); ITGB4PB gene; Nucleolus; Translation; Carrier Proteins; Cell Nucleolus; Consensus Sequence; Dimerization; Eukaryotic Initiation Factors; GA-Binding Protein Transcription Factor; HeLa Cells; Humans; Intermediate Filament Proteins; Promoter Regions, Genetic; Protein Binding; Ribosomes; Sequence Deletion; Trans-Activators; Gene Expression Regulation; Transcription, Genetic; Structural Biology; Biophysics; Biochemistry; Molecular Biology; Genetics; Cell Biology
Settore BIO/06 - Anatomia Comparata e Citologia
FEBS LETTERS
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/492829
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