Eukaryotic initiation factor 6 (eIF6), an essential protein important in ribosome biosynthesis and assembly, was identified as an interacting partner of the β-catenin C terminus in the yeast two-hybrid assay. Independent studies identified Drosophila eIF6 (DeIF6) in a genetic screen designed to detect new genes involved in the regulation of the Wnt/Wg (wingless) pathway. Ectopic expression of DeIF6 in wing discs results in a Wg phenotype. Expression of eIF6 in adenomatous polyposis coli (APC)-mutant colon cancer cells, which express high levels of active β-catenin, showed that eIF6 selectively inhibits the Wnt pathway at the level of β-catenin protein independently of proteasomal degradation. Incorporation of radiolabeled amino acids into β-catenin was selectively decreased in cells that overexpressed eIF6. A similar inverse relationship of the two proteins was observed in the APC min/+ mouse intestine, in which β-catenin levels are very high. Taken together these data reveal a link between eIF6 and Wnt signaling, perhaps at the level of ribosome recycling on β-catenin mRNA.
Eukaryotic initiation factor 6 selectively regulates Wnt signaling and β-catenin protein synthesis / Y. Ji, S. Shah, K. Soanes, M.N. Islam, B. Hoxter, S. Biffo, T. Heslip, S. Byers. - In: ONCOGENE. - ISSN 0950-9232. - 27:6(2008), pp. 755-762.
|Titolo:||Eukaryotic initiation factor 6 selectively regulates Wnt signaling and β-catenin protein synthesis|
|Parole Chiave:||β-catenin; APC; Drosophila; eIF6; Wingless; Wnt; Adenomatous Polyposis Coli Protein; Amino Acids; Animals; Drosophila; Drosophila Proteins; Intestines; Mice; Mice, Mutant Strains; Peptide Initiation Factors; Protein Biosynthesis; Protein Structure, Tertiary; RNA, Messenger; Signal Transduction; Wings, Animal; Wnt Proteins; beta Catenin; Molecular Biology; Genetics; Cancer Research|
|Settore Scientifico Disciplinare:||Settore BIO/06 - Anatomia Comparata e Citologia|
|Data di pubblicazione:||2008|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1038/sj.onc.1210667|
|Appare nelle tipologie:||01 - Articolo su periodico|