Purpose: We demonstrated that Hsa-miR-567 expression is significantly downregulated in poor prognosis breast cancer, compared to better prognosis breast cancer, having a role in the control of cell proliferation and migration by regulating KPNA4 gene. Methods and results: In this study, based on our previously published in silico results, we proved both in vitro (cell line studies) and ex vivo (clinical studies), that Hsa-miR-567 expression is significantly downregulated in breast cancer with poor prognosis when compared to breast cancer with better prognosis. More intriguingly, we demonstrated that the ectopic expression of Hsa-miR-567 in poor prognosis breast cancer cell line strongly inhibits in vitro cell proliferation and migration. Furthermore, we showed in vivo that breast cancer cells, stably expressing Hsa-miR-567, xenografted in mouse, reduce tumor growth ability. Consistently, we found that karyopherin 4 (KPNA4), predicted target gene of Hsa-miR-567 as identified by our in silico analysis, is upregulated in highly aggressive MDA-MB-231 breast cancer cell line and patient tissues with poor prognosis with respect to good prognosis. Conclusions: Our results suggest a potential role of Hsa-miR-567 as a novel prognostic biomarker for BC and as regulator of KPNA4.
MicroRNA-567 dysregulation contributes to carcinogenesis of breast cancer, targeting tumor cell proliferation, and migration / G. Bertoli, C. Cava, C. Diceglie, C. Martelli, G. Rizzo, F. Piccotti, L. Ottobrini, I. Castiglioni. - In: BREAST CANCER RESEARCH AND TREATMENT. - ISSN 0167-6806. - 161:3(2017 Feb), pp. 605-616.
|Titolo:||MicroRNA-567 dysregulation contributes to carcinogenesis of breast cancer, targeting tumor cell proliferation, and migration|
OTTOBRINI, LUISA (Penultimo)
|Parole Chiave:||Biomarker; Breast cancer; MicroRNA/miRNA; Prognosis; Proliferation; Oncology; Cancer Research|
|Settore Scientifico Disciplinare:||Settore MED/50 - Scienze Tecniche Mediche Applicate|
Settore MED/36 - Diagnostica per Immagini e Radioterapia
|Data di pubblicazione:||feb-2017|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1007/s10549-016-4079-2|
|Appare nelle tipologie:||01 - Articolo su periodico|