ATP-binding cassette (ABC) transporters are a large superfamily of proteins responsible for the ATP-dependent translocation of a wide variety of different en-dogenous and exogenous toxicants across cellular membranes. We used a PCR-based approach to isolate from Botrytis cinerea a DNA fragment that proved be an ABC type transporter gene following sequencing and Southern blot analyses. This gene, named BcatrO, was characterized by RT-PCR expression and knock-out mutant phenotype analysis. Treatment of B. cinerea with H2O2 and methyl viologen enhanced transcription of BcatrO. An increase of the gene transcripts was also observed in the early stages of pathogenesis after inoculation of the fungus to bean leaves. BcatrO knock-out mutants of B. cinerea were more sensitive than the wild type both to H2O2 and fungicide fenpiclonil, but displayed the same virulence. The possible role of BcatrO in a mechanism of resistance of B. cinerea to reactive oxygen species (ROS) or their products and in pleiotropic drug resistance phenomena is discussed.

Further studies on the etiological agents of carpinus betulus decline / M. Saracchi, F. Rocchi, S. Quaroni. - In: JOURNAL OF PLANT PATHOLOGY. - ISSN 1125-4653. - 90:Suppl. 2(2008), pp. 453-453.

Further studies on the etiological agents of carpinus betulus decline

M. Saracchi;F. Rocchi;S. Quaroni
2008

Abstract

ATP-binding cassette (ABC) transporters are a large superfamily of proteins responsible for the ATP-dependent translocation of a wide variety of different en-dogenous and exogenous toxicants across cellular membranes. We used a PCR-based approach to isolate from Botrytis cinerea a DNA fragment that proved be an ABC type transporter gene following sequencing and Southern blot analyses. This gene, named BcatrO, was characterized by RT-PCR expression and knock-out mutant phenotype analysis. Treatment of B. cinerea with H2O2 and methyl viologen enhanced transcription of BcatrO. An increase of the gene transcripts was also observed in the early stages of pathogenesis after inoculation of the fungus to bean leaves. BcatrO knock-out mutants of B. cinerea were more sensitive than the wild type both to H2O2 and fungicide fenpiclonil, but displayed the same virulence. The possible role of BcatrO in a mechanism of resistance of B. cinerea to reactive oxygen species (ROS) or their products and in pleiotropic drug resistance phenomena is discussed.
ABC transporters; Botrytis cinerea; Hydrogen peroxide; PCR; Reactive oxygen species
Settore AGR/12 - Patologia Vegetale
JOURNAL OF PLANT PATHOLOGY
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/49217
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