Staphylococcus pseudintermedius is an opportunistic pathogen of dogs and cats. A high-resolution melting analysis (HRMA) protocol was designed and tested on 42 clinical isolates with known fluoroquinolone (FQ) susceptibility and gyrA codon 84 and grlA codon 80 mutation status. The HRMA approach was able to discriminate between FQ-sensitive and FQ-resistant strains and confirmed previous reports that the main mutation site associated with FQ resistance in S. pseudintermedius is located at position 251 (Ser84Leu) of gyrA.

High-resolution melting analysis of gyrA codon 84 and grlA codon 80 mutations conferring resistance to fluoroquinolones in Staphylococcus pseudintermedius isolates from canine clinical samples / M. Loiacono, P.A. Martino, F. Albonico, F. Dell’Orco, M. Ferretti, S. Zanzani, M. Mortarino. - In: JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION. - ISSN 1040-6387. - (2017 May). [Epub ahead of print] [10.1177/1040638717712330]

High-resolution melting analysis of gyrA codon 84 and grlA codon 80 mutations conferring resistance to fluoroquinolones in Staphylococcus pseudintermedius isolates from canine clinical samples

M. Loiacono
Primo
;
P.A. Martino
Secondo
;
F. Albonico;F. Dell’Orco;M. Ferretti;S. Zanzani
Penultimo
;
M. Mortarino
2017

Abstract

Staphylococcus pseudintermedius is an opportunistic pathogen of dogs and cats. A high-resolution melting analysis (HRMA) protocol was designed and tested on 42 clinical isolates with known fluoroquinolone (FQ) susceptibility and gyrA codon 84 and grlA codon 80 mutation status. The HRMA approach was able to discriminate between FQ-sensitive and FQ-resistant strains and confirmed previous reports that the main mutation site associated with FQ resistance in S. pseudintermedius is located at position 251 (Ser84Leu) of gyrA.
Staphylococcus pseudintermedius; fluoroquinolone resistance; high-resolution melting
Settore VET/06 - Parassitologia e Malattie Parassitarie degli Animali
Settore VET/05 - Malattie Infettive degli Animali Domestici
mag-2017
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/492006
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