Most plant species rely on seeds for their dispersal and survival under unfavorable environmental conditions. Seeds are characterized by their low moisture content and significantly reduced metabolic activities. During the maturation phase, seeds accumulate storage reserves and become desiccation-tolerant and dormant. Growth is resumed after release of dormancy and the occurrence of favorable environmental conditions. Here we show that embryonic cotyledon nuclei of Arabidopsis thaliana seeds have a significantly reduced nuclear size, which is established at the beginning of seed maturation. In addition, the chromatin of embryonic cotyledon nuclei from mature seeds is highly condensed. Nuclei regain their size and chromatin condensation level during germination. The reduction in nuclear size is controlled by the seed maturation regulator ABSCISIC ACID-INSENSITIVE 3, and the increase during germination requires two predicted nuclear matrix proteins, LITTLE NUCLEI 1 and LITTLE NUCLEI 2. Our results suggest that the specific properties of nuclei in ripe seeds are an adaptation to desiccation, independent of dormancy. We conclude that the changes in nuclear size and chromatin condensation in seeds are independent, developmentally controlled processes.

Seed maturation in Arabidopsis thaliana is characterized by nuclear size reduction and increased chromatin condensation / M. Van Zanten, M.A. Koini, R. Geyer, Y. Liu, V. Brambilla, D. Bartels, M. Koornneef, P. Fransz, W.J.J. Soppe. - In: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. - ISSN 0027-8424. - 108:50(2011 Dec 13), pp. 20219-20224.

Seed maturation in Arabidopsis thaliana is characterized by nuclear size reduction and increased chromatin condensation

V. Brambilla;
2011

Abstract

Most plant species rely on seeds for their dispersal and survival under unfavorable environmental conditions. Seeds are characterized by their low moisture content and significantly reduced metabolic activities. During the maturation phase, seeds accumulate storage reserves and become desiccation-tolerant and dormant. Growth is resumed after release of dormancy and the occurrence of favorable environmental conditions. Here we show that embryonic cotyledon nuclei of Arabidopsis thaliana seeds have a significantly reduced nuclear size, which is established at the beginning of seed maturation. In addition, the chromatin of embryonic cotyledon nuclei from mature seeds is highly condensed. Nuclei regain their size and chromatin condensation level during germination. The reduction in nuclear size is controlled by the seed maturation regulator ABSCISIC ACID-INSENSITIVE 3, and the increase during germination requires two predicted nuclear matrix proteins, LITTLE NUCLEI 1 and LITTLE NUCLEI 2. Our results suggest that the specific properties of nuclei in ripe seeds are an adaptation to desiccation, independent of dormancy. We conclude that the changes in nuclear size and chromatin condensation in seeds are independent, developmentally controlled processes.
English
chromatin organization; seed development; seed germination; seed imbibition; adaptation, physiological; arabidopsis; arabidopsis proteins; chromatin; cotyledon; cytogenetic analysis; desiccation; plant dormancy; seeds; cell nucleus size; multidisciplinary
Settore BIO/01 - Botanica Generale
Articolo
Esperti anonimi
Ricerca di base
Pubblicazione scientifica
13-dic-2011
The National Academy of Sciences of the USA
108
50
20219
20224
6
Pubblicato
Periodico con rilevanza internazionale
scopus
crossref
pubmed
Aderisco
info:eu-repo/semantics/article
Seed maturation in Arabidopsis thaliana is characterized by nuclear size reduction and increased chromatin condensation / M. Van Zanten, M.A. Koini, R. Geyer, Y. Liu, V. Brambilla, D. Bartels, M. Koornneef, P. Fransz, W.J.J. Soppe. - In: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. - ISSN 0027-8424. - 108:50(2011 Dec 13), pp. 20219-20224.
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Article (author)
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M. Van Zanten, M.A. Koini, R. Geyer, Y. Liu, V. Brambilla, D. Bartels, M. Koornneef, P. Fransz, W.J.J. Soppe
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/489425
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