Introduction Both obesity and periodontal diseases (PD: gingivitis, periodontitis) represent a source of low-grade systemic inflammation with higher gestational risks. We showed increased inflammation and oxidant levels in saliva (S) of obese (OB) vs normalweight (NW) pregnant women, heightened by PD. Here we quantified 758 microRNAs (miRNA) in S of these women. Methods 29 singleton pregnancies (14 NW, BMI 18-24.9; 15 OB, BMI≥30) with no obstetric complications were studied at 3rd trimester. Periodontal status was assessed by oral clinical examination. Unstimulated saliva was collected and 758 miRNA from microvescicle fractions were quantified by QuantStudio OpenArray. Fold change (FC) of each miRNA was calculated as the ratio between the relative quantity in OBvsNW or in PD vs oral-healthy (OH) women. Raw p-values, from univariate and multivariate linear regression models, were adjusted for multiple testing by controlling the false discovery rate (FDR). Informatics investigation was performed by FireFly (AbCam) and TargetScan Database. Results 11/15 OB and 8/14 NW had PD, confirming higher PD rate in OB. Prepregnancy BMI was inversely correlated to placental efficiency (PlE, fetal/placental weight) that among PD was significantly decreased in OBvsNW (p 0.047). 334/754 miRNA were expressed in at least 1 subject. 103 were differentially expressed (FC≤0.5 or ≥2) in OBvsNW and 91 in PDvsOH. miR483-5p was significantly lower in PDvsOH in univariate analysis and adjusted for BMI (FDR p 0.028 and 0.043). Only in NW miR330 was significantly lower in PDvsOH (FDR p 0.114), while in OB it maintained low levels both in PD and OH. Conclusions We found different S miRNA expression profiles in both OB and PD that might reflect molecular systemic changes in these previously described high-risk pregnancies. Moreover, miR483-5p was significantly decreased in PDvsOH. This miRNA regulates genes involved in fat metabolism and cell growth/differentiation i.e. IGF2 and TGFβ1. miR330, regulating genes involved in placentation and fetal growth i.e. AKT, also had different expression in PDvsOH, but only in NW. This suggests a role of the obese environment in its regulation with a synergistic action of OB and PD to miR330 downregulation, possibly leading to higher risk pregnancies, as suggested by lower PlE in women presenting both PD and OB.
Obesity and Periodontal Diseases in Pregnancy : miRNAome in saliva / C. Mandò, S. Abati, G.M. Anelli, L. Dioni, C. Novielli, C. Favero, L. Cantone, M. Cardellicchio, I. Cetin, V. Bollati. - In: REPRODUCTIVE SCIENCES. - ISSN 1933-7191. - 24:suppl. 1(2017 Mar 15), pp. 249A-249A. (Intervento presentato al 64. convegno Annual Scientific Meeting of the Society for Reproductive Investigation tenutosi a Orlando nel 2017).
Obesity and Periodontal Diseases in Pregnancy : miRNAome in saliva
C. MandòPrimo
;G.M. Anelli;L. Dioni;C. Novielli;C. Favero;L. Cantone;M. Cardellicchio;I. CetinPenultimo
;V. BollatiUltimo
2017
Abstract
Introduction Both obesity and periodontal diseases (PD: gingivitis, periodontitis) represent a source of low-grade systemic inflammation with higher gestational risks. We showed increased inflammation and oxidant levels in saliva (S) of obese (OB) vs normalweight (NW) pregnant women, heightened by PD. Here we quantified 758 microRNAs (miRNA) in S of these women. Methods 29 singleton pregnancies (14 NW, BMI 18-24.9; 15 OB, BMI≥30) with no obstetric complications were studied at 3rd trimester. Periodontal status was assessed by oral clinical examination. Unstimulated saliva was collected and 758 miRNA from microvescicle fractions were quantified by QuantStudio OpenArray. Fold change (FC) of each miRNA was calculated as the ratio between the relative quantity in OBvsNW or in PD vs oral-healthy (OH) women. Raw p-values, from univariate and multivariate linear regression models, were adjusted for multiple testing by controlling the false discovery rate (FDR). Informatics investigation was performed by FireFly (AbCam) and TargetScan Database. Results 11/15 OB and 8/14 NW had PD, confirming higher PD rate in OB. Prepregnancy BMI was inversely correlated to placental efficiency (PlE, fetal/placental weight) that among PD was significantly decreased in OBvsNW (p 0.047). 334/754 miRNA were expressed in at least 1 subject. 103 were differentially expressed (FC≤0.5 or ≥2) in OBvsNW and 91 in PDvsOH. miR483-5p was significantly lower in PDvsOH in univariate analysis and adjusted for BMI (FDR p 0.028 and 0.043). Only in NW miR330 was significantly lower in PDvsOH (FDR p 0.114), while in OB it maintained low levels both in PD and OH. Conclusions We found different S miRNA expression profiles in both OB and PD that might reflect molecular systemic changes in these previously described high-risk pregnancies. Moreover, miR483-5p was significantly decreased in PDvsOH. This miRNA regulates genes involved in fat metabolism and cell growth/differentiation i.e. IGF2 and TGFβ1. miR330, regulating genes involved in placentation and fetal growth i.e. AKT, also had different expression in PDvsOH, but only in NW. This suggests a role of the obese environment in its regulation with a synergistic action of OB and PD to miR330 downregulation, possibly leading to higher risk pregnancies, as suggested by lower PlE in women presenting both PD and OB.File | Dimensione | Formato | |
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