Continuous or primary epithelial cell lines have been extensively used to study the mammary gland immune response, but they are constituted by a single cell population. Our aim was to test whether an explant of heifer gland, where the tissue structure is maintained, might be a valid model to investigate the innate immune response to infection. The study was carried out on 2 mm3-sections of heifer udders, in 2 consecutive trials, using LPS or LTA in the first trial and two different concentrations of Staphylococcus aureus (Staph. aureus) in the second. Treated and untreated sections were collected after 1 h, 3 h and 6 h incubation; in the first trial, a final time-point at 18 h was considered. The mRNA expression of TNFα, IL-1β, IL-6, IL-8 and LAP was analyzed by quantitative real-time PCR. Histological examination showed well-preserved morphology of the tissue, and apoptosis only showed a slight, not significant increase throughout the experiment. IL-1β and IL-6 were significantly up-regulated, in response to LPS or Staph. aureus, while TNF-α and IL-8 significantly increased only under LPS treatment. LAP expression showed a significant late increase when stimulated by LPS. The immunochemical staining of the sections demonstrated a higher number of T lymphocytes within the alveolar epithelium, in comparison with interstitial localization. Since the explants belonged to pubertal non-pregnant heifers, T cells may be regarded as resident cells, suggesting their participation in the regulation of mammary homeostasis. Therefore, applying our model would give new insights in the investigation of udder pathophysiology.
An explant of heifer mammary gland to study the immune response of the organ / G. Magro, T.A.L. Brevini, M. De Maglie, G. Minozzi, E. Scanziani, R. Piccinini. - In: RESEARCH IN VETERINARY SCIENCE. - ISSN 0034-5288. - 114(2017 Oct), pp. 44-50.
|Titolo:||An explant of heifer mammary gland to study the immune response of the organ|
MAGRO, GIADA (Primo)
SCANZIANI, EUGENIO (Penultimo)
PICCININI, RENATA (Corresponding)
|Parole Chiave:||Bovine mammary gland; Explant; Innate immunity; Mastitis; Veterinary (all)|
|Settore Scientifico Disciplinare:||Settore AGR/17 - Zootecnica Generale e Miglioramento Genetico|
Settore VET/06 - Parassitologia e Malattie Parassitarie degli Animali
Settore VET/05 - Malattie Infettive degli Animali Domestici
|Data di pubblicazione:||ott-2017|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.rvsc.2017.03.002|
|Appare nelle tipologie:||01 - Articolo su periodico|