The expression of genes requiring finely tuned control is regulated by a posttranscriptional mechanism involving mRNA A + U-rich elements (AREs) cooperating with ARE-binding proteins (AUBPs) in modulation of mRNA stability. We reported previously that an ARE in the bcl-2 mRNA 3'-untranslated region (3'-UTR) had destabilizing activity and was involved in bcl-2 downregulation during apoptosis in vitro. Here we demonstrate that the bcl-2 ARE complexes with a number of specific AUBPs, whose pattern undergoes changes following application of apoptotic stimuli. The caspase inhibitor Z-VAD-fmk strongly attenuates both bcl-2 mRNA decay and bcl-2 AUBP pattern changes elicited by apoptotic stimuli, indicating the involvement of bcl-2 AUBPs in bcl-2 mRNA stability control.
|Titolo:||Apoptosis is associated with modifications of bcl-2 mRNA AU-binding proteins|
|Parole Chiave:||apoptosis ; bcl-2 ; gene expression ; posttranscriptional control ; AU-rich elements ; AU-binding proteins|
|Settore Scientifico Disciplinare:||Settore BIO/14 - Farmacologia|
|Data di pubblicazione:||ott-2001|
|Digital Object Identifier (DOI):||10.1006/bbrc.2001.5700|
|Appare nelle tipologie:||01 - Articolo su periodico|