We sought to characterise circulating and tissue tumour biomarkers of patients who developed early-stage non-small cell lung cancer (NSCLC) during long-term follow-up of a chemoprevention trial (NCT00321893). Materials and Methods: Blood and sputum samples were collected from 202 high-risk asymptomatic individuals with CT-detected stable lung nodules. Real-time PCR was performed on plasma to quantify free circulating DNA. Baseline serum was investigated with a previously validated test based on 13 circulating miRNAs (miR-Test). Promoter methylation status of p16, RASSF1a and RARâ2 and telomerase activity were assessed in sputum samples. DNA was extracted from each tumour developed during follow-up and subjected to a mutation survey using the LungCarta panel on the Sequenom MassARRAY platform. Results: During follow-up (9 years) six individuals underwent surgery for stage I NSCLC with a median time of disease onset of 20.5 months. MiR-Test scores were positive (range: 0.14-7.24) in four out of six baseline pre-disease onset sera. No association was identified between free circulating DNA or sputum biomarkers and disease onset. All tumours harboured at least one somatic mutation in well-known cancer genes, including KRAS (n = 4), BRAF (n = 1), and TP53 (n = 3). Conclusion: Circulating miRNA tests may represent valuable tools to detect clinically-silent tumours. Early-stage lung adenocarcinomas harbour recurrent genetic events similar to those described in advanced-stage NSCLCs.
Circulating and tissue biomarkers in early-stage non-small cell lung cancer / C. Fumagalli, F. Bianchi, P.R. Raviele, D. Vacirca, G. Bertalot, C. Rampinelli, M. Lazzeroni, B. Bonanni, G. Veronesi, N. Fusco, M. Barberis, E. Guerini-Rocco. - In: ECANCERMEDICALSCIENCE. - ISSN 1754-6605. - 11(2017), pp. 717.1-717.10.
|Titolo:||Circulating and tissue biomarkers in early-stage non-small cell lung cancer|
BIANCHI, FABRIZIO (Secondo)
GUERINI ROCCO, ELENA (Ultimo)
|Parole Chiave:||circulating biomarker; early detection; non-small cell lung cancer; somatic mutation; oncology; cancer research|
|Settore Scientifico Disciplinare:||Settore MED/08 - Anatomia Patologica|
|Data di pubblicazione:||2017|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.3332/ecancer.2017.717|
|Appare nelle tipologie:||01 - Articolo su periodico|