The quantitative detection of some peculiar analytes under physiological conditions is of critical importance in clinical diagnosis. This is especially true for neurotransmitters because of their key role in behavior expression, during excessive oxidative stress events, for the early diagnosis of neurodegenerative diseases (such as Parkinson) and some kinds of tumors. In this context, a nanocomposite electrode with a sandwich structure combining the properties of silver nanoparticles and a nanotitania photoactive layer was used for the electroanalytical detection, by differential pulse voltammetry, of three neurotransmitters: dopamine, norepinephrine, and serotonin. The three analytes were determined at low detection limits (around 0.03 μM) also in the presence of conventional interferents, such as uric and ascorbic acids. The fouling of the electrode surface, a major drawback in the detection of these analytes, was overcome by irradiating the device with UVA light, restoring the initial sensor sensitivity. Dopamine, norepinephrine, and serotonin were determined also in simulated biological matrices: liquor (artificially reproduced cerebrospinal fluid) and serum. Moreover, the contemporaneous detection of dopamine and norepinephrine in simulated human urine solutions was also demonstrated, representing the first step towards clinical applications of the methodology for the development of an integrated monitoring system [1-3]. Acknowledgments This work has been supported by Fondazione Cariplo (Italy), grant no. 2014-1285. [1] G. Soliveri, V. Pifferi, G. Panzarasa, S. Ardizzone, G. Cappelletti, D. Meroni, K. Sparnacci, L. Falciola, Analyst 2015, 140, 1486 [2] V. Pifferi, G. Soliveri, G. Panzarasa, S. Ardizzone, G. Cappelletti, D. Meroni, L. Falciola, RSC Advances 2015, 5, 71210 [3] V. Pifferi, G. Soliveri, G. Panzarasa, G. Cappelletti, D. Meroni, L. Falciola, Anal. Bioanal. Chem. 2016, doi:10.1007/s00216-016-9539-3
Photorenewable nanostructured electroanalytical sensor for neurotransmitters detection in body fluid mimics / L. Falciola. ((Intervento presentato al convegno Nanomedicine Symposium CEN@UniMiB: Towards Translation and European Networking tenutosi a Milano nel 2016.
Photorenewable nanostructured electroanalytical sensor for neurotransmitters detection in body fluid mimics
L. FalciolaPrimo
2016
Abstract
The quantitative detection of some peculiar analytes under physiological conditions is of critical importance in clinical diagnosis. This is especially true for neurotransmitters because of their key role in behavior expression, during excessive oxidative stress events, for the early diagnosis of neurodegenerative diseases (such as Parkinson) and some kinds of tumors. In this context, a nanocomposite electrode with a sandwich structure combining the properties of silver nanoparticles and a nanotitania photoactive layer was used for the electroanalytical detection, by differential pulse voltammetry, of three neurotransmitters: dopamine, norepinephrine, and serotonin. The three analytes were determined at low detection limits (around 0.03 μM) also in the presence of conventional interferents, such as uric and ascorbic acids. The fouling of the electrode surface, a major drawback in the detection of these analytes, was overcome by irradiating the device with UVA light, restoring the initial sensor sensitivity. Dopamine, norepinephrine, and serotonin were determined also in simulated biological matrices: liquor (artificially reproduced cerebrospinal fluid) and serum. Moreover, the contemporaneous detection of dopamine and norepinephrine in simulated human urine solutions was also demonstrated, representing the first step towards clinical applications of the methodology for the development of an integrated monitoring system [1-3]. Acknowledgments This work has been supported by Fondazione Cariplo (Italy), grant no. 2014-1285. [1] G. Soliveri, V. Pifferi, G. Panzarasa, S. Ardizzone, G. Cappelletti, D. Meroni, K. Sparnacci, L. Falciola, Analyst 2015, 140, 1486 [2] V. Pifferi, G. Soliveri, G. Panzarasa, S. Ardizzone, G. Cappelletti, D. Meroni, L. Falciola, RSC Advances 2015, 5, 71210 [3] V. Pifferi, G. Soliveri, G. Panzarasa, G. Cappelletti, D. Meroni, L. Falciola, Anal. Bioanal. Chem. 2016, doi:10.1007/s00216-016-9539-3
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