Adducin regulates tubular absorption of sodium by modulating the expression levels of the sodium-potassium-ATPase in renal tubular cells. Adducin is a candidate gene in the pathogenesis of hypertension. Yeast two hybrid screen showed a specific interaction between human alpha Adducin and the regulatory factor for X box (RFX-1), a nuclear protein that down regulates the expression of several proteins in non neuronal cells. The interaction was confirmed in cells through co-immunoprecipitation and colocalization experiments. The binding of alpha Adducin to RFX-I and their nuclear co-localization suggests that Adducin can have a role in modulating the transcriptional regulating activity of RFX-I.
|Titolo:||RFX-1, a putative alpha Adducin interacting protein in a human kidney library|
|Parole Chiave:||Cellule COS, Sistema a doppio ibrido, DNA binding proteins|
|Settore Scientifico Disciplinare:||Settore MED/14 - Nefrologia|
|Data di pubblicazione:||21-nov-2005|
|Digital Object Identifier (DOI):||10.1016/j.febslet.2005.10.040|
|Appare nelle tipologie:||01 - Articolo su periodico|