HIV-1-specific mucosal IgA antibodies may correlate with protection in highly exposed but uninfected individuals, but have been detected at highly variable levels in HIV-1-infected individuals. To determine the best assays for detection of IgA antibodies in mucosal samples, rectal washes from 16 HIV-1-infected and 14 uninfected individuals were distributed to six laboratories experienced in detection of mucosal antibodies. Assays for HIV-1-specific IgA and IgG were performed in a blinded fashion by each of the laboratories using modifications of ELISA and chemiluminescence-enhanced Western blotting. Rectal washes contained easily detectable total IgA levels that did not differ between HIV-1-infected and uninfected groups. Irrespective of the assay used, HIV-1-specific IgA antibodies were absent in most samples; only one laboratory identified a higher frequency of positive samples from HIV-1-infected than uninfected individuals. In spite of 10-fold lower levels of total IgG than IgA, all but one laboratory identified HIV-1-specific IgG in most rectal washes of HIV-1-infected individuals. Comparable and readily detectable levels of influenza-specific IgA antibodies were present in nasal, salivary, and rectal secretions from both HIV-1-infected and uninfected individuals. These observations suggest a selective alteration in the production of HIV-1-specific IgA antibodies in HIV-1-infected individuals.
Detection of mucosal antibodies in HIV type 1-infected individuals / P.F. Wright, P.A. Kozlowski, G.K. Rybczyk, P. Goepfert, H.F. Staats, T.C. Vancott, D. Trabattoni, E. Sannella, J. Mestecky. - In: AIDS RESEARCH AND HUMAN RETROVIRUSES. - ISSN 0889-2229. - 18:17(2002), pp. 1291-1300. ((Intervento presentato al convegno AIDS Vaccine tenutosi a Philadelphia nel 2001.
|Titolo:||Detection of mucosal antibodies in HIV type 1-infected individuals|
|Parole Chiave:||human-immunodeficiency-virus; genital-tract secretions; infected individuals; seronegative partens; immune-responses; IGA antibodies; secretory IGA; sex workers; induction; women|
|Settore Scientifico Disciplinare:||Settore MED/04 - Patologia Generale|
Settore MED/17 - Malattie Infettive
Settore BIO/13 - Biologia Applicata
Settore MED/46 - Scienze Tecniche di Medicina di Laboratorio
|Data di pubblicazione:||2002|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1089/088922202320886334|
|Appare nelle tipologie:||01 - Articolo su periodico|