Severe muscle fibrosis is the endpoint of many chronic myopathies. Identification of factors that regulate fibrosis is important for understanding its pathogenesis and for developing anti-fibrotic treatments that prevent muscle destruction. We have developed an in vitro model for screening potential anti-fibrotic agents. The model consists of three-dimensional clusters (nodules) of fibroblasts derived from Duchenne muscular dystrophy (DMD) muscle. The primary fibroblasts spontaneously and quickly form nodules resembling fibrotic foci (cells plus extracellular matrix) when grown on a solid substrate. We tested the anti-fibrotic action of suramin, decorin, and spironolactone (all with established anti-fibrotic activity) on the model. All three agents significantly reduced nodule number, and spironolactone and suramin significantly reduced nodule diameter. Nodule secretion of soluble collagen was also significantly reduced by decorin and spironolactone treatment, whereas suramin had no significant effect. Collagen I and fibronectin protein expression was significantly reduced in the culture medium of control and DMD fibroblasts by spironolactone treatment, but not by decorin and suramin treatment. Finally, in DMD fibroblast monolayers, collagen deposition was significantly reduced by all three agents. Spironolactone significantly reduced collagen I and fibronectin transcript levels, whereas decorin reduced only fibronectin. Our in vitro model of fibrogenesis has thus revealed differing anti-fibrotic effects in the three anti-fibrotic agents tested. It therefore appears as a useful and sensitive system for the testing of anti-fibrotic drugs and could be adapted for the high-throughput screening of new anti-fibrotic molecules.
|Titolo:||Duchenne muscular dystrophy fibroblast nodules : a cell-based assay for screening anti-fibrotic agents|
|Parole Chiave:||Cell culture (Human); Decorin; Fibroblast; Muscle fibrosis; Spironolactone; Suramin; Biological Assay; Blotting, Western; Collagen; Decorin; Enzyme-Linked Immunosorbent Assay; Fibroblasts; Fibronectins; Fibrosis; Gene Expression Regulation; Humans; Muscular Dystrophy, Duchenne; RNA, Messenger; Spironolactone; Suramin; Drug Evaluation, Preclinical; 2734; Cell Biology; Histology|
|Settore Scientifico Disciplinare:||Settore BIO/11 - Biologia Molecolare|
|Data di pubblicazione:||giu-2013|
|Digital Object Identifier (DOI):||10.1007/s00441-013-1601-2|
|Appare nelle tipologie:||01 - Articolo su periodico|