The effects of methyl jasmonate (MeJA), an elicitor of plant defense mechanisms, on the biosynthesis of diosgenin, a steroidal saponin, were investigated in six fenugreek (Trigonella foenum-graecum) varieties (Gujarat Methi-2, Kasuri-1, Kasuri-2, Pusa Early Branching, Rajasthan Methi and Maharashtra Methi-5). Treatment with 0.01% MeJA increased diosgenin levels, in 12 days old seedlings, from 0.5%-0.9% to 1.1%-1.8%. In addition, MeJA upregulated the expression of two pivotal genes of the mevalonate pathway, the metabolic route leading to diosgenin: 3-hydroxy-3-methylglutaryl-CoA reductase (HMG) and sterol-3-β-glucosyl transferase (STRL). In particular, MeJA increased the expression of HMG and STRL genes by 3.2- and 22.2-fold, respectively, in the Gujarat Methi-2 variety, and by 25.4- and 28.4-fold, respectively, in the Kasuri-2 variety. Therefore, MeJA may be considered a promising elicitor for diosgenin production by fenugreek plants.
|Titolo:||Elicitation of diosgenin production in Trigonella foenum-graecum (fenugreek) seedlings by methyl jasmonate|
IRITI, MARCELLO (Corresponding)
|Parole Chiave:||3-hydroxy-3-methylglutaryl-CoA reductase; Elicitors; Mevalonate pathway; Plant innate immunity; Steroidal saponins; Sterol-3- β -glucosyl transferase; Acetates; Biomass; Biosynthetic Pathways; Cyclopentanes; Diosgenin; Electrophoresis, Agar Gel; Gene Expression Regulation, Plant; Genes, Essential; Genes, Plant; Oxylipins; Seedlings; Trigonella; Physical and Theoretical Chemistry; Organic Chemistry; Spectroscopy; Inorganic Chemistry; Catalysis; Molecular Biology; Computer Science Applications1707 Computer Vision and Pattern Recognition|
|Settore Scientifico Disciplinare:||Settore AGR/12 - Patologia Vegetale|
|Data di pubblicazione:||2015|
|Digital Object Identifier (DOI):||10.3390/ijms161226208|
|Appare nelle tipologie:||01 - Articolo su periodico|