The aim of this study was to determine whether the intrinsic mechanism of apoptosis is involved in the death of germ cells in Robertsonian (Rb) heterozygous adult male mice. Testes from 5-month-old Rb heterozygous CD1 × Milano 11 mice were obtained and compared with those from homozygous CD1 (2n = 40) and Milano II (2n = 24) mice. For histological evaluation of apoptosis, TUNEL labelling and immunohistochemistry were used to locallise Bax and cytochrome c. Expression of calbindin D28k (CB), an anti-apoptotic molecule, was also analysed by immunohistochemistry and immunoblotting. Testicular ultrastructure was visualised by electron microscopy. Morphology and cell associations were abnormal in the Rb heterozygous sermiferous epithelium. An intense apoptotic process was observed in tubules at stage XII, mainly in metaphase spermatocytes. Metaphase spermatocytes also showed Bax and cytochrome c redistributions. Mitochondria relocated close to the paranuclear region of spermatocytes. CB was mainly expressed in metaphase spermatocytes, but also in pachytene spermatocytes, spermatids and Sertoli cells at stage XII. The co-localisation of CB and TUNEL labelling was very limited. Sixty per cent of metaphase spermatocytes were apoptotic and calbindin negative, while 40% were calbindin positive without signs of apoptosis. Ten per cent of the Bax- and cytochrome c-positive cells were also calbindin positive. These data suggest that apoptosis of the germ cells in heterozygous mice occurs, at least in part, through a mitochondrial-dependent mechanism. Calbindin overexpression might prevent or reduce the apoptosis of germ cells caused by Rb heterozygosity, which could partially explain the subfertility of these mice.

A mitochondrial mechanism is involved in apoptosis of Robertsonian mouse male germ cells / V. Merico, G.D. de Barboza, C. Vasco, R. Ponce, V. Rodriguez, S. Garagna, N.T. de Talamoni. - In: REPRODUCTION. - ISSN 1470-1626. - 135:6(2008), pp. 797-804. [10.1530/REP-07-0466]

A mitochondrial mechanism is involved in apoptosis of Robertsonian mouse male germ cells

C. Vasco;
2008

Abstract

The aim of this study was to determine whether the intrinsic mechanism of apoptosis is involved in the death of germ cells in Robertsonian (Rb) heterozygous adult male mice. Testes from 5-month-old Rb heterozygous CD1 × Milano 11 mice were obtained and compared with those from homozygous CD1 (2n = 40) and Milano II (2n = 24) mice. For histological evaluation of apoptosis, TUNEL labelling and immunohistochemistry were used to locallise Bax and cytochrome c. Expression of calbindin D28k (CB), an anti-apoptotic molecule, was also analysed by immunohistochemistry and immunoblotting. Testicular ultrastructure was visualised by electron microscopy. Morphology and cell associations were abnormal in the Rb heterozygous sermiferous epithelium. An intense apoptotic process was observed in tubules at stage XII, mainly in metaphase spermatocytes. Metaphase spermatocytes also showed Bax and cytochrome c redistributions. Mitochondria relocated close to the paranuclear region of spermatocytes. CB was mainly expressed in metaphase spermatocytes, but also in pachytene spermatocytes, spermatids and Sertoli cells at stage XII. The co-localisation of CB and TUNEL labelling was very limited. Sixty per cent of metaphase spermatocytes were apoptotic and calbindin negative, while 40% were calbindin positive without signs of apoptosis. Ten per cent of the Bax- and cytochrome c-positive cells were also calbindin positive. These data suggest that apoptosis of the germ cells in heterozygous mice occurs, at least in part, through a mitochondrial-dependent mechanism. Calbindin overexpression might prevent or reduce the apoptosis of germ cells caused by Rb heterozygosity, which could partially explain the subfertility of these mice.
mus-musculus-domesticus; seminiferous epithelium; house mice; vitamin-D; spermatogenesis; expression; death; testis; spermatocytes; rat
Settore BIO/13 - Biologia Applicata
2008
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/474881
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