¿DESIGN AND SYNTHESIS OF MODIFIED PEPTIDE NUCLEIC ACID (PNAS) WITH IMPROVED PHYSICO-CHEMICAL PROPERTIES FOR DNA, RNA AND MICRO RNA TARGETING¿

The present dissertation is divided into four chapters. Chapter one deals with the introduction to Peptide Nucleic Acid (PNA) its properties and its applications. The second chapter deals with the synthesis of modified PNAs with and without fluorescein isothiourea and its kinetic studies by using stopped-flow technique. The third chapter deals with the design, synthesis and characterization of functionalized PNAs with heterobifunctional linker and their conjugation with magnetic nanoparticles (MNPs) for miRNA targeting. The fourth chapter deals with design and synthesis of modified thymine monomer PNA and homothymine decamer PNA sequence with gold(I) complexes for cellular imaging.