This study aimed to collect, characterize ultrafine particles (UFP) generated from the combustion of wood pellets and logs (softwood and hardwood) and to evaluate their pro-inflammatory effects in THP-1 and A549 cells. Both cell lines responded to UFP producing interleukin-8 (IL-8), with wood log UFP being more active compared to pellet UFP. With the exception of higher effect observed with beech wood log UFP in THP-1, the ability of soft or hard woods to induce IL-8 release was similar. In addition, on weight mass, IL-8 release was similar or lower compared to diesel exhaust particles (DEP), arguing against higher biological activity of smaller size particles. UFP-induced IL-8 could be reduced by SB203580, indicating a role of p38MAPK activation in IL-8 production. The higher activity of beech wood log UFP in THP-1 was not due to higher uptake or endotoxin contamination. Qualitatively different protein adsorption profiles were observed, with less proteins bound to beech UFP compared to conifer UFP or DEP, which may provide higher intracellular availability of bioactive components, i.e. levoglucosan and galactosan, toward which THP-1 were more responsive compared to A549 cells. These results contribute to our understanding of particles emitted by domestic appliances and their biological effects.

Insights on wood combustion generated proinflammatory ultrafine particles (UFP) / E. Corsini, S. Ozgen, A. Papale, V. Galbiati, G. Lonati, P. Fermo, L. Corbella, G. Valli, V. Bernardoni, M. Dell'Acqua, S. Becagli, D. Caruso, R. Vecchi, C.L. Galli, M. Marinovich. - In: TOXICOLOGY LETTERS. - ISSN 0378-4274. - 266(2017 Jan 15), pp. 74-84. [10.1016/j.toxlet.2016.12.005]

Insights on wood combustion generated proinflammatory ultrafine particles (UFP)

E. Corsini
;
A. Papale;V. Galbiati;P. Fermo;G. Valli;V. Bernardoni;D. Caruso;R. Vecchi;C.L. Galli
Penultimo
;
M. Marinovich
Ultimo
2017

Abstract

This study aimed to collect, characterize ultrafine particles (UFP) generated from the combustion of wood pellets and logs (softwood and hardwood) and to evaluate their pro-inflammatory effects in THP-1 and A549 cells. Both cell lines responded to UFP producing interleukin-8 (IL-8), with wood log UFP being more active compared to pellet UFP. With the exception of higher effect observed with beech wood log UFP in THP-1, the ability of soft or hard woods to induce IL-8 release was similar. In addition, on weight mass, IL-8 release was similar or lower compared to diesel exhaust particles (DEP), arguing against higher biological activity of smaller size particles. UFP-induced IL-8 could be reduced by SB203580, indicating a role of p38MAPK activation in IL-8 production. The higher activity of beech wood log UFP in THP-1 was not due to higher uptake or endotoxin contamination. Qualitatively different protein adsorption profiles were observed, with less proteins bound to beech UFP compared to conifer UFP or DEP, which may provide higher intracellular availability of bioactive components, i.e. levoglucosan and galactosan, toward which THP-1 were more responsive compared to A549 cells. These results contribute to our understanding of particles emitted by domestic appliances and their biological effects.
Cytokine; Inflammation; Protein corona; UFP; Wood; Toxicology
Settore BIO/14 - Farmacologia
Settore CHIM/01 - Chimica Analitica
Settore FIS/07 - Fisica Applicata(Beni Culturali, Ambientali, Biol.e Medicin)
Settore BIO/10 - Biochimica
Settore CHIM/12 - Chimica dell'Ambiente e dei Beni Culturali
15-dic-2016
Article (author)
File in questo prodotto:
File Dimensione Formato  
TOBICUP_TOXLETT_2017.pdf

accesso riservato

Tipologia: Publisher's version/PDF
Dimensione 1.81 MB
Formato Adobe PDF
1.81 MB Adobe PDF   Visualizza/Apri   Richiedi una copia
TOXLETTERS_2017_pre-print_CORSINI.pdf

accesso aperto

Tipologia: Pre-print (manoscritto inviato all'editore)
Dimensione 363.7 kB
Formato Adobe PDF
363.7 kB Adobe PDF Visualizza/Apri
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/470820
Citazioni
  • ???jsp.display-item.citation.pmc??? 3
  • Scopus 24
  • ???jsp.display-item.citation.isi??? 23
social impact