The aim of this research is to study the influence of protein kinase C δ on the nuclear phospholipids metabolism. Murine and human melanoma cells, in which overexpression of protein kinase δ was induced, were used. After purification of the nuclei, the phosphatidylcholine-dependent phospholipase C, sphingomyelin-synthase, and sphingomyelinase activities were measured. The results showed that the nuclear sphingomyelin-synthase activity increased and sphingomyelinase activity decreased in the protein kinase C δ overexpressive cells with respect to the controls. As a consequence, the ceramide pool decreased and diacylglycerol pool increased; this effect was not due to the phosphatidylcholine-dependent phospholipase C activity that did not change. The inhibition of sphingomyelinase could be due to protein kinase C δ as well as to existence of a sort of nuclear self-regulation between sphingomyelin-synthase and sphingomyelinase. The possible role of nuclear sphingomyelin-synthase in cell proliferation is discussed.
|Titolo:||Nuclear sphyingomyelin-synthase and protein kinase C delta in melanoma cells|
LA PORTA, CATERINA (Secondo)
|Parole Chiave:||Ceramide; Diacylglycerol; Melanoma; Sphingomyelin-synthase; Sphingomyelinase|
|Settore Scientifico Disciplinare:||Settore MED/04 - Patologia Generale|
|Data di pubblicazione:||2005|
|Appare nelle tipologie:||01 - Articolo su periodico|