The aim of this research is to study the influence of protein kinase C δ on the nuclear phospholipids metabolism. Murine and human melanoma cells, in which overexpression of protein kinase δ was induced, were used. After purification of the nuclei, the phosphatidylcholine-dependent phospholipase C, sphingomyelin-synthase, and sphingomyelinase activities were measured. The results showed that the nuclear sphingomyelin-synthase activity increased and sphingomyelinase activity decreased in the protein kinase C δ overexpressive cells with respect to the controls. As a consequence, the ceramide pool decreased and diacylglycerol pool increased; this effect was not due to the phosphatidylcholine-dependent phospholipase C activity that did not change. The inhibition of sphingomyelinase could be due to protein kinase C δ as well as to existence of a sort of nuclear self-regulation between sphingomyelin-synthase and sphingomyelinase. The possible role of nuclear sphingomyelin-synthase in cell proliferation is discussed.
Nuclear sphyingomyelin-synthase and protein kinase C delta in melanoma cells / E. Albi, C. A. M. La Porta, S. Cataldi, M. Viola Magni. - In: ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS. - ISSN 0003-9861. - 438:2(2005), pp. 156-161.
Nuclear sphyingomyelin-synthase and protein kinase C delta in melanoma cells
C. A. M. La PortaSecondo
;
2005
Abstract
The aim of this research is to study the influence of protein kinase C δ on the nuclear phospholipids metabolism. Murine and human melanoma cells, in which overexpression of protein kinase δ was induced, were used. After purification of the nuclei, the phosphatidylcholine-dependent phospholipase C, sphingomyelin-synthase, and sphingomyelinase activities were measured. The results showed that the nuclear sphingomyelin-synthase activity increased and sphingomyelinase activity decreased in the protein kinase C δ overexpressive cells with respect to the controls. As a consequence, the ceramide pool decreased and diacylglycerol pool increased; this effect was not due to the phosphatidylcholine-dependent phospholipase C activity that did not change. The inhibition of sphingomyelinase could be due to protein kinase C δ as well as to existence of a sort of nuclear self-regulation between sphingomyelin-synthase and sphingomyelinase. The possible role of nuclear sphingomyelin-synthase in cell proliferation is discussed.Pubblicazioni consigliate
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