Polyurethene (PU) catheters were coated with 3-(trimethoxysilyl)-propyldimethyloctadecylammonium chloride (QAS) by means of a multistep process which involved a vapor phase plasma-induced graft-polymerization of acrylic acid (AAc). The AAc coating, whose stability in aqueous media was assessed by immersion in Phospate Buffer Saline (PBS), was characterized by means of Attenuated Total Reflectance Fourier Transform Infrared (ATR/FTIR) spectroscopy. Moreover, the COOH surface density was evaluated by a colorimetric assay with Methylene Blue. Carrying a negative charge at neutral pH, AAc coatings were proficient in positively charged molecules (like QAS) adsorption. ATR/FTIR spectroscopy and a colorimetric assay with Bromophenol Blue allowed us to verify the presence and the uniformity of the QAS coating on the PU catheters and the positive effect of the AAc graft-polymerization on the QAS adsorption. Morphological characterization of the QAS-modified catheters was performed by means of Atomic Force Microscopy (AFM). QAS-coated catheters displayed in vitro antimicrobial activity against Gram-negative Escherichia coli bacterial cells.
Development of antibacterial quaternary ammonium silane coatings on polyurethane catheters / S. Zanini, A. Polissi, E.A. Maccagni, E.C. Dell'Orto, C. Liberatore, C. Riccardi. - In: JOURNAL OF COLLOID AND INTERFACE SCIENCE. - ISSN 0021-9797. - 451(2015), pp. 78-84.
|Titolo:||Development of antibacterial quaternary ammonium silane coatings on polyurethane catheters|
POLISSI, ALESSANDRA (Secondo)
|Parole Chiave:||Acrylic acid; AFM; ATR/FTIR; Escherichia coli; Plasma-induced graft-polymerization; Quaternary ammonium silane; Acrylates; Anti-Bacterial Agents; Catheters; Coated Materials, Biocompatible; Escherichia coli; Escherichia coli Infections; Humans; Microbial Sensitivity Tests; Polyurethanes; Quaternary Ammonium Compounds; Silanes; Surface Properties; Electronic, Optical and Magnetic Materials; Biomaterials; Surfaces, Coatings and Films; Colloid and Surface Chemistry|
|Settore Scientifico Disciplinare:||Settore BIO/19 - Microbiologia Generale|
Settore FIS/01 - Fisica Sperimentale
|Data di pubblicazione:||2015|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.jcis.2015.04.007|
|Appare nelle tipologie:||01 - Articolo su periodico|