Chemokine receptors are differentially expressed on leukocyte subpopulations dictating their ability to migrate both in physiological and pathological conditions. Their expression is modulated during leukocyte differentiation and maturation and they can be used as markers to identify and characterize the frequency and the activation state of leukocytes present in a tissue. Here, we will describe flow cytometry approaches to detect chemokine receptors identifying subpopulations of circulating monocytes and neutrophils.
Flow Cytometry Detection of Chemokine Receptors for the Identification of Murine Monocyte and Neutrophil Subsets / O. Bonavita, M. Massara, A. Anselmo, P. Somma, H. Brühl, M. Mack, M. Locati, R. Bonecchi (METHODS IN ENZYMOLOGY). - In: Chemokines / [a cura di] T.M. Handel. - Amsterdam : Academic Press, 2016. - ISBN 978-0-12-802171-2. - pp. 441-456
Titolo: | Flow Cytometry Detection of Chemokine Receptors for the Identification of Murine Monocyte and Neutrophil Subsets | |
Autori: | BONAVITA, ORNELLA (Primo) MASSARA, MATTEO (Secondo) LOCATI, MASSIMO (Penultimo) BONECCHI, RAFFAELLA (Ultimo) | |
Parole Chiave: | Chemokine receptors; Chemokines; Flow cytometry; Monocytes; Neutrophils; Biochemistry; Molecular Biology | |
Settore Scientifico Disciplinare: | Settore BIO/13 - Biologia Applicata | |
Data di pubblicazione: | 2016 | |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1016/bs.mie.2015.09.011 | |
Tipologia: | Book Part (author) | |
Appare nelle tipologie: | 03 - Contributo in volume |
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