In regenerative medicine, human cord blood-derived multipotent mesenchymal stromal cells (CBMSCs) stand out for their biological peculiarities demonstrated in in vitro and in vivo preclinical studies. Here, we present our 9-year experience for the consistent isolation of CBMSCs. Although nearly one CB unit out of two retains the potential to give rise to MSC colonies, only 46% of them can be cultured till low passages (P≥4), but one-fourth of those reaches even higher passages (P≥8). Subsequent characterization for morphological, clonal, differentiation, and proliferation properties revealed two divergent CBMSC behaviors. In particular, a cumulative population doublings cut-off (CPD=15) was identified that undoubtedly distinguishes two growth curves, and different degrees of commitment toward osteogenesis were observed. These data clearly show the existence of at least two distinct CBMSC subsets: one mainly short-living and less proliferative (SL-CBMSCs), the other long-living, with higher growth rate, and, very importantly, with significantly (P≤0.01) longer telomere (LL-CBMSCs). Moreover, significant differences in the immunoprofile before seeding were found among CB units giving rise to LL-CBMSCs or SL-CBMSCs or showing no colony formation. Finally, all the aforementioned results provided a peculiar and useful set of parameters potentially predictive for CBMSC culture outcome.
|Titolo:||Dissection of the cord blood stromal component reveals predictive parameters for culture outcome|
LAVAZZA, CRISTIANA (Secondo)
|Parole Chiave:||Cell Culture Techniques; Fetal Blood; Humans; Mesenchymal Stromal Cells; Cell Differentiation; Osteogenesis; Hematology; Developmental Biology; Cell Biology|
|Settore Scientifico Disciplinare:||Settore BIO/15 - Biologia Farmaceutica|
|Data di pubblicazione:||1-gen-2015|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1089/scd.2014.0160|
|Appare nelle tipologie:||01 - Articolo su periodico|