Collagen-made materials and scaffolds are the most promising in tissue engineering applications, since they simultaneously offer biocompatibility and optimal mechanical performances. In this work we used common marine invertebrates (echinoderms) as an innovative and low-cost source of native intact collagen fibrils to develop ECM equivalents. Collagen fibrils were isolated from connective tissues of different echinoderms (sea urchins, starfish, sea cucumber), using putative by-products of the food industry or fishery. These fibrils were used to prepare films and/or 3D scaffolds which were characterized and compared in terms of ultrastructure and biomechanics. In vitro biocompatibility tests were performed using mesenchymal stem cells isolated from horse peripheral blood; preliminary in vivo tests were assessed by subcutaneous implantation of the scaffold in rabbits and CAM tests for angiogenesis evaluation. The obtained fibrillar matrices strictly mimicked the native ECM and displayed mechanical performances in the range of natural tendon and skin. In vitro tests indicated that such substrates are not toxic for mammalian cells, which, after an initial “adaptation” stage, were also able to actively proliferate. In vivo tests suggest encouraging results related to biocompatibility and angiogenetic response, although further investigations are necessary. Overall echinoderm-derived collagenous matrices have the potential to be used for tendon or skin regenerative medicine.
Echinoderms as sustainable source of collagen for innovative applications in regenerative medicine / M. Sugni, C. Di Benedetto, T. Martinello, C. Ferrario, L. Leggio, A. Barbaglio, C. Gomiero, F. Bonasoro, M. Patruno, M.D. Candia Carnevali. ((Intervento presentato al 6. convegno Bilateral Seminar Italy-Japan tenutosi a Palermo nel 2014.
Echinoderms as sustainable source of collagen for innovative applications in regenerative medicine
M. SugniPrimo
;C. Di BenedettoSecondo
;C. Ferrario;A. Barbaglio;F. Bonasoro;M.D. Candia CarnevaliUltimo
2014
Abstract
Collagen-made materials and scaffolds are the most promising in tissue engineering applications, since they simultaneously offer biocompatibility and optimal mechanical performances. In this work we used common marine invertebrates (echinoderms) as an innovative and low-cost source of native intact collagen fibrils to develop ECM equivalents. Collagen fibrils were isolated from connective tissues of different echinoderms (sea urchins, starfish, sea cucumber), using putative by-products of the food industry or fishery. These fibrils were used to prepare films and/or 3D scaffolds which were characterized and compared in terms of ultrastructure and biomechanics. In vitro biocompatibility tests were performed using mesenchymal stem cells isolated from horse peripheral blood; preliminary in vivo tests were assessed by subcutaneous implantation of the scaffold in rabbits and CAM tests for angiogenesis evaluation. The obtained fibrillar matrices strictly mimicked the native ECM and displayed mechanical performances in the range of natural tendon and skin. In vitro tests indicated that such substrates are not toxic for mammalian cells, which, after an initial “adaptation” stage, were also able to actively proliferate. In vivo tests suggest encouraging results related to biocompatibility and angiogenetic response, although further investigations are necessary. Overall echinoderm-derived collagenous matrices have the potential to be used for tendon or skin regenerative medicine.Pubblicazioni consigliate
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