The developmental programme of the pistil is under the control of both auxin and cytokinin. Crosstalk between these factors converges on regulation of the auxin carrier PIN-FORMED 1 (PIN1). Here, we show that in the triple transcription factor mutant cytokinin response factor 2 (crf2) crf3 crf6 both pistil length and ovule number were reduced. PIN1 expression was also lower in the triple mutant and the phenotypes could not be rescued by exogenous cytokinin application. pin1 complementation studies using genomic PIN1 constructs showed that the pistil phenotypes were only rescued when the PCRE1 domain, to which CRFs bind, was present. Without this domain, pin mutants resemble the crf2 crf3 crf6 triple mutant, indicating the pivotal role of CRFs in auxin-cytokinin crosstalk.
Cytokinin response factors integrate auxin and cytokinin pathways for female reproductive organ development / M. Cucinotta, S. Manrique, A. Guazzotti, N.E. Quadrelli, M.A. Mendes, E. Benkova, L. Colombo. - In: DEVELOPMENT. - ISSN 1477-9129. - 143:23(2016 Dec), pp. 4419-4424.
|Titolo:||Cytokinin response factors integrate auxin and cytokinin pathways for female reproductive organ development|
CUCINOTTA, MARA (Primo)
COLOMBO, LUCIA (Ultimo)
|Parole Chiave:||CRFs; ovule primordia; PIN1; pistil; plant hormones|
|Settore Scientifico Disciplinare:||Settore BIO/01 - Botanica Generale|
|Data di pubblicazione:||dic-2016|
|Data ahead of print / Data di stampa:||ott-2016|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1242/dev.143545|
|Appare nelle tipologie:||01 - Articolo su periodico|