Many efforts have been pursued to promote recovery of function of the spinal cord after injuries. The pharmacological approach has been recently flanked by cellular methods, and the use of stem cells has even more recently been a new tool for spinal cord therapy. The aim of the study was the assessment of the effects of adult neural stem cell (NSCs) and undifferentiated embryonic stem (ES) cells transplantation in a mouse model of spinal cord injury (SCI). The injury was performed by mean of the Infinite Horizon Device, that allows the generation of reproducible traumatic lesion to the cord. We administered green fluorescent labeled (GFP-)NSCs or the Q-tracker labeled ES by intravenous injection. We report that the two treatments significantly improved recovery of hind limb function and greatly attenuated secondary degeneration; however, NSCs administration promoted a better recovery than ES treatments. After one months, about 2% of total i.v. administered NSCs or 0.1 % of the transplanted ES cells were still present at the site of spinal cord injury. In both cases the transplanted cells survived almost undifferentiated; thus the positive effect cannot be ascribed to damaged tissue substitution. Only the NSCs homing to the injury site triggered within 48 hours a large increase of the expression of neurotrophic factors and chemokynes. One week after transplantation exogenous GFP-NSCs still retained their proliferation potential and produced neurospheres when recovered from the lesion site and cultured in vitro. At later time GFP-NSC were phagocytated by macrophages. The ES transplanted animals showed a better preservation of ventral myelin at the lesion site than PBS treated mice, but no changes of neuroprotective or inflammatory cytokines were observed. Furthermore the number of macrophages was much lower in the ES transplanted animals when compared with the vehicle treated mice. No teratomas formation after ES transplantation was observed in the span of time that the animals underwent this study; but several ES bound to the uninjured sacral cord portion. In conclusion, both the cellular approaches with NSC and ES cells induced a significant functional and morphological recovery after spinal cord injury

Spinal cord injury : a comparison of neural stem cell and embryonic stem cell treatments / D. Bottai, L. Madaschi, D. Cigognini, R. Adami, E. Nicora, A.M. Di Giulio, A. Gorio. - In: JOURNAL OF NEUROTRAUMA. - ISSN 0897-7151. - 25:7(2008 Jul 07), pp. 889-889. (Intervento presentato al 26. convegno Annual Neurotrauma Society Symposium tenutosi a Orlando nel 2008).

Spinal cord injury : a comparison of neural stem cell and embryonic stem cell treatments

D. Bottai
Primo
;
L. Madaschi
Secondo
;
D. Cigognini;R. Adami;E. Nicora;A.M. Di Giulio
Penultimo
;
A. Gorio
Ultimo
2008

Abstract

Many efforts have been pursued to promote recovery of function of the spinal cord after injuries. The pharmacological approach has been recently flanked by cellular methods, and the use of stem cells has even more recently been a new tool for spinal cord therapy. The aim of the study was the assessment of the effects of adult neural stem cell (NSCs) and undifferentiated embryonic stem (ES) cells transplantation in a mouse model of spinal cord injury (SCI). The injury was performed by mean of the Infinite Horizon Device, that allows the generation of reproducible traumatic lesion to the cord. We administered green fluorescent labeled (GFP-)NSCs or the Q-tracker labeled ES by intravenous injection. We report that the two treatments significantly improved recovery of hind limb function and greatly attenuated secondary degeneration; however, NSCs administration promoted a better recovery than ES treatments. After one months, about 2% of total i.v. administered NSCs or 0.1 % of the transplanted ES cells were still present at the site of spinal cord injury. In both cases the transplanted cells survived almost undifferentiated; thus the positive effect cannot be ascribed to damaged tissue substitution. Only the NSCs homing to the injury site triggered within 48 hours a large increase of the expression of neurotrophic factors and chemokynes. One week after transplantation exogenous GFP-NSCs still retained their proliferation potential and produced neurospheres when recovered from the lesion site and cultured in vitro. At later time GFP-NSC were phagocytated by macrophages. The ES transplanted animals showed a better preservation of ventral myelin at the lesion site than PBS treated mice, but no changes of neuroprotective or inflammatory cytokines were observed. Furthermore the number of macrophages was much lower in the ES transplanted animals when compared with the vehicle treated mice. No teratomas formation after ES transplantation was observed in the span of time that the animals underwent this study; but several ES bound to the uninjured sacral cord portion. In conclusion, both the cellular approaches with NSC and ES cells induced a significant functional and morphological recovery after spinal cord injury
Settore BIO/14 - Farmacologia
7-lug-2008
Neurotrama Society
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/46281
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