Human placental mesenchymal stromal cells (pMSCs) have never been investigated in intrauterine growth restriction (IUGR). We characterized cells isolated from placental membranes and the basal disc of six IUGR and five physiological placentas. Cell viability and proliferation were assessed every 7 days during a 6-week culture. Expression of hematopoietic, stem, endothelial, and mesenchymal markers was evaluated by flow cytometry. We characterized the multipotency of pMSCs and the expression of genes involved in mitochondrial content and function. Cell viability was high in all samples, and proliferation rate was lower in IUGR compared with control cells. All samples presented a starting heterogeneous population, shifting during culture toward homogeneity for mesenchymal markers and occurring earlier in IUGR than in controls. In vitro multipotency of IUGR-derived pMSCs was restricted because their capacity for adipocyte differentiation was increased, whereas their ability to differentiate toward endothelial cell lineagewasdecreased. Mitochondrial content and function were higher in IUGR pMSCs than controls, possibly indicating a shift from anaerobic to aerobic metabolism, with the loss of the metabolic characteristics that are typical of undifferentiated multipotent cells.
Impaired Angiogenic Potential of Human Placental Mesenchymal Stromal Cells in Intrauterine Growth Restriction / C. Mandò, P. Razini, C. Novielli, G.M. Anelli, M. Belicchi, S. Erratico, S. Banfi, M. Meregalli, A. Tavelli, M. Baccarin, A. Rolfo, S. Motta, Y. Torrente, I. Cetin. - In: STEM CELLS TRANSLATIONAL MEDICINE. - ISSN 2157-6564. - 5:4(2016 Apr 05), pp. 451-463.
|Titolo:||Impaired Angiogenic Potential of Human Placental Mesenchymal Stromal Cells in Intrauterine Growth Restriction|
MANDO', CHIARA (Primo)
TORRENTE, YVAN (Corresponding)
CETIN, IRENE (Ultimo)
|Parole Chiave:||Intrauterine growth restriction; Mesenchymal stromal cells; Placenta; Pregnancy; Stem cells; Case-Control Studies; Cell Differentiation; Cell Movement; Cells, Cultured; Colony-Forming Units Assay; Endothelium, Vascular; Female; Fetal Growth Retardation; Humans; Mesenchymal Stromal Cells; Microvessels; Placenta; Pregnancy; Neovascularization, Physiologic; Cell Biology; Developmental Biology|
|Settore Scientifico Disciplinare:||Settore MED/40 - Ginecologia e Ostetricia|
Settore BIO/11 - Biologia Molecolare
Settore BIO/13 - Biologia Applicata
Settore MED/26 - Neurologia
|Data di pubblicazione:||5-apr-2016|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.5966/sctm.2015-0155|
|Appare nelle tipologie:||01 - Articolo su periodico|