The Pax3 and c-met genes are necessary for the development of tongue, diaphragm, and limb muscles. These hypaxial muscles derive from precursors that migrate out of the ventrolateral lip of the somites at occipital, cervical, and limb levels. In this work, we re-examined primary myogenesis in c-met signaling mutants using a skeletal muscle-specific lacZ transgene (Mlc3f-nlacZ-2E). This strategy allowed us to identify precisely the shoulder, limb, tongue, and dermal muscles that need Met for development and to confirm that the morphological structure of epaxial and body wall muscles was normal, even in the most severe c-met mutant. Surprisingly, however, X-gal staining showed that, in this mutant, hyoid arch-derived facial muscles were either reduced or absent, thus revealing that Met also contributes to the development of muscles in the head.
Analysis of Mlc-lacZ met mutants highlights the essential function of met for migratory precursors of hypaxial muscles and reveals a role for met in the development of hyoid arch-derived facial muscles / C. Prunotto, T. Crepaldi, P.E. Forni, A. Ieraci, R.G. Kelly, S. Tajbakhsh, M. Buckingham, C. Ponzetto. - In: DEVELOPMENTAL DYNAMICS. - ISSN 1058-8388. - 231:3(2004 Nov 23), pp. 582-591.
|Titolo:||Analysis of Mlc-lacZ met mutants highlights the essential function of met for migratory precursors of hypaxial muscles and reveals a role for met in the development of hyoid arch-derived facial muscles|
|Parole Chiave:||c-met; Head muscles;hypaxial muscles; migratory muscle precursors; pax3; splotch; animals; cell lineage; cell movement; facial muscles; gene expression regulation, developmental; in situ hybridization; kinetics; mesoderm; mice; mice, mutant strains; mice, transgenic; muscle development; muscles; proto-oncogene proteins c-met; transgenes; beta-galactosidase; lac operon; mutation; developmental biology; cell biology|
|Settore Scientifico Disciplinare:||Settore BIO/14 - Farmacologia|
Settore BIO/10 - Biochimica
Settore BIO/11 - Biologia Molecolare
Settore BIO/13 - Biologia Applicata
|Data di pubblicazione:||23-nov-2004|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1002/dvdy.20177|
|Appare nelle tipologie:||01 - Articolo su periodico|