cAMP effects have been initially attributed to protein kinase A (PKA) activation. Subsequently, two exchange proteins directly activated by cAMP (Epac1/2) have been identified as cAMP targets. Aim of this study was to investigate cAMP effects in pancreatic-NET (P-NET) and bronchial carcinoids and in corresponding cell lines (QGP-1 and H727) on cell proliferation and adhesion and to determine PKA and Epac role in mediating these effects. We found that cAMP increased cyclin D1 expression in P-NET and QGP-1 cells, whereas it had opposite effects on bronchial carcinoids and H727 cells and it promoted cell adhesion in QGP-1 and H727 cells. These effects are mimicked by Epac and PKA specific analogs, activating the small GTPase Rap1. In conclusion, we demonstrated that cAMP exerted divergent effects on proliferation and promoted cell adhesion of different neuroendocrine cell types, these effects being mediated by both Epac and PKA and involving the same effector GTPase Rap1.

cAMP effects in neuroendocrine tumors : the role of Epac and PKA in cell proliferation and adhesion / E. Vitali, V. Cambiaghi, A. Spada, A. Tresoldi, A. Zerbi, E. Peverelli, C. Carnaghi, G. Mantovani, A.G. Lania. - In: EXPERIMENTAL CELL RESEARCH. - ISSN 0014-4827. - 339:2(2015), pp. 241-251. [10.1016/j.yexcr.2015.11.011]

cAMP effects in neuroendocrine tumors : the role of Epac and PKA in cell proliferation and adhesion

A. Spada;A. Tresoldi;E. Peverelli;G. Mantovani
Penultimo
;
2015

Abstract

cAMP effects have been initially attributed to protein kinase A (PKA) activation. Subsequently, two exchange proteins directly activated by cAMP (Epac1/2) have been identified as cAMP targets. Aim of this study was to investigate cAMP effects in pancreatic-NET (P-NET) and bronchial carcinoids and in corresponding cell lines (QGP-1 and H727) on cell proliferation and adhesion and to determine PKA and Epac role in mediating these effects. We found that cAMP increased cyclin D1 expression in P-NET and QGP-1 cells, whereas it had opposite effects on bronchial carcinoids and H727 cells and it promoted cell adhesion in QGP-1 and H727 cells. These effects are mimicked by Epac and PKA specific analogs, activating the small GTPase Rap1. In conclusion, we demonstrated that cAMP exerted divergent effects on proliferation and promoted cell adhesion of different neuroendocrine cell types, these effects being mediated by both Epac and PKA and involving the same effector GTPase Rap1.
CAMP; CAMP-dependent Protein Kinase A (PKA); Exchange proteins directly activated by cAMP (Epac); Neuroendocrine tumors; Ras-like GTPase Rap1; Cell Adhesion; Cyclic AMP; Cyclic AMP-Dependent Protein Kinases; Guanine Nucleotide Exchange Factors; Humans; Neuroendocrine Tumors; Tumor Cells, Cultured; Cell Proliferation; Cell Biology
Settore MED/13 - Endocrinologia
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/456322
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