Peripheral blood mononuclear cells (PBMC) were collected from a patient with clozapine-resistant (also known as “super-refractory”) Schizophrenia. iPSCs were established with a non-integrating Sendai Virus-based reprogramming system. A footprint-free hiPSC line was characterized to express the main endogenous pluripotency markers and a normal karyotype. Cells showed pluripotency competency by giving rise to progeny of differentiated cells belonging to the three germ layers. This hiPSC line represents a valuable tool to obtain mature, pathology-relevant neuronal populations in vitro that are suitable to investigate the molecular background of the schizophrenic disorder and the resultant patients' response to treatments.

Generation and characterization of an induced pluripotent stem cell (iPSC) line from a patient with clozapine-resistant Schizophrenia / F. Marsoner, M. Marcatili, T. Karnavas, D. Bottai, A. D'Agostino, S. Scarone, L. Conti. - In: STEM CELL RESEARCH. - ISSN 1873-5061. - (2016). [Epub ahead of print]

Generation and characterization of an induced pluripotent stem cell (iPSC) line from a patient with clozapine-resistant Schizophrenia

M. Marcatili
Secondo
;
D. Bottai;A. D'Agostino;S. Scarone
Penultimo
;
2016

Abstract

Peripheral blood mononuclear cells (PBMC) were collected from a patient with clozapine-resistant (also known as “super-refractory”) Schizophrenia. iPSCs were established with a non-integrating Sendai Virus-based reprogramming system. A footprint-free hiPSC line was characterized to express the main endogenous pluripotency markers and a normal karyotype. Cells showed pluripotency competency by giving rise to progeny of differentiated cells belonging to the three germ layers. This hiPSC line represents a valuable tool to obtain mature, pathology-relevant neuronal populations in vitro that are suitable to investigate the molecular background of the schizophrenic disorder and the resultant patients' response to treatments.
Settore BIO/14 - Farmacologia
Settore BIO/10 - Biochimica
Settore BIO/09 - Fisiologia
Settore BIO/13 - Biologia Applicata
2016
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/455110
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