Objective. Transforming growth factor beta(3) (TGF-beta(3)) is a potent suppressor of human hematopoietic progenitor cells. In this article, we compare the activity of TGF-beta(3) on highly purified CD34(+) cells and more immature CD34(+)DR(-) cells from chronic myelogenous leukemia (CML) patients in chronic phase and normal donors. Materials and Methods. Primitive hematopoietic progenitors mere stimulated in liquid cultures and clonogenic assays by early-acting growth factors such as stem cell factor (SCF) and interleukin 11 (IL-11) and the intermediate-late-acting stimulating factors IL-3, granulocyte-macrophage colony-stimulating factor, and erythropoietin. Molecular analysis of bcr/abl mRNA was performed on single CML colonies by nested reverse transcriptase polymerase chain reaction. Moreover, cell cycle analysis and assessment of apoptosis of normal and leukemic CD34(+) cells were performed by propidium iodide (PI) alone and simultaneous staining with annexin V and PI, respectively. Results. The colony-forming efficiency of CML CD34(+) cells was generally inhibited by more than 90% regardless of whether the colony-stimulating factors were used alone or combined. When compared to normal CD34(+) cells, leukemic cells mere significantly more suppressed in 6 of 8 culture conditions. The inhibitory effect of TGF-beta(3) on CD34(+) cells was exerted within the first 24 hours of incubation as demonstrated by short-term preincubation followed by IL-3- and SCF-stimulated colony assays. Evaluation of bcr/abl transcript on residual CML colonies incubated with TGF-beta(3) demonstrated a small subset of neoplastic CD34(+) cells unresponsive to the inhibitory effect of the study cytokine, TGF-beta(3) demonstrated a greater inhibitory activity on primitive CD34(+)DR(-) cells than on more mature CD34(+) cells. Again, CML CD34(+)DR(-) cells were significantly more inhibited by TGF-beta(3) than their normal counterparts in 3 of 8 culture conditions. Kinetic analysis performed on CD34(+) cells showed that TGF-beta induces cell cycle arrest in G(1) phase. However, this mechanism of action is shared by normal and leukemic cells. Conversely, TGF-beta(3) preferentially triggered the programmed cell death of CML CD34(+) cells without increasing the proportion of leukemic cells coexpressing CD95 (Fas receptor), and this effect was not reversed by functional blockade of Fas receptor. Conclusion. We demonstrate that TGF-beta(3) exerts a potent suppressive effect on CML cells that is partly mediated hy Fas-independent apoptosis.
Transforming growth factor beta(3) inhibits chronic myelogenous leukemia hematopoiesis by inducing Fas-independent apoptosis / M. Fogli, C. Carlo-Stella, A. Curti, M. Ratta, P.L. Tazzari, E. Regazzi, S. Colla, A.M. Santucci, S. Tura, R. Lemoli. - In: EXPERIMENTAL HEMATOLOGY. - ISSN 0301-472X. - 28:7(2000), pp. 775-783. [10.1016/S0301-472X(00)00173-9]
Transforming growth factor beta(3) inhibits chronic myelogenous leukemia hematopoiesis by inducing Fas-independent apoptosis
C. Carlo-StellaSecondo
;
2000
Abstract
Objective. Transforming growth factor beta(3) (TGF-beta(3)) is a potent suppressor of human hematopoietic progenitor cells. In this article, we compare the activity of TGF-beta(3) on highly purified CD34(+) cells and more immature CD34(+)DR(-) cells from chronic myelogenous leukemia (CML) patients in chronic phase and normal donors. Materials and Methods. Primitive hematopoietic progenitors mere stimulated in liquid cultures and clonogenic assays by early-acting growth factors such as stem cell factor (SCF) and interleukin 11 (IL-11) and the intermediate-late-acting stimulating factors IL-3, granulocyte-macrophage colony-stimulating factor, and erythropoietin. Molecular analysis of bcr/abl mRNA was performed on single CML colonies by nested reverse transcriptase polymerase chain reaction. Moreover, cell cycle analysis and assessment of apoptosis of normal and leukemic CD34(+) cells were performed by propidium iodide (PI) alone and simultaneous staining with annexin V and PI, respectively. Results. The colony-forming efficiency of CML CD34(+) cells was generally inhibited by more than 90% regardless of whether the colony-stimulating factors were used alone or combined. When compared to normal CD34(+) cells, leukemic cells mere significantly more suppressed in 6 of 8 culture conditions. The inhibitory effect of TGF-beta(3) on CD34(+) cells was exerted within the first 24 hours of incubation as demonstrated by short-term preincubation followed by IL-3- and SCF-stimulated colony assays. Evaluation of bcr/abl transcript on residual CML colonies incubated with TGF-beta(3) demonstrated a small subset of neoplastic CD34(+) cells unresponsive to the inhibitory effect of the study cytokine, TGF-beta(3) demonstrated a greater inhibitory activity on primitive CD34(+)DR(-) cells than on more mature CD34(+) cells. Again, CML CD34(+)DR(-) cells were significantly more inhibited by TGF-beta(3) than their normal counterparts in 3 of 8 culture conditions. Kinetic analysis performed on CD34(+) cells showed that TGF-beta induces cell cycle arrest in G(1) phase. However, this mechanism of action is shared by normal and leukemic cells. Conversely, TGF-beta(3) preferentially triggered the programmed cell death of CML CD34(+) cells without increasing the proportion of leukemic cells coexpressing CD95 (Fas receptor), and this effect was not reversed by functional blockade of Fas receptor. Conclusion. We demonstrate that TGF-beta(3) exerts a potent suppressive effect on CML cells that is partly mediated hy Fas-independent apoptosis.File | Dimensione | Formato | |
---|---|---|---|
1-s2.0-S0301472X00001739-main.pdf
accesso riservato
Tipologia:
Publisher's version/PDF
Dimensione
1.1 MB
Formato
Adobe PDF
|
1.1 MB | Adobe PDF | Visualizza/Apri Richiedi una copia |
Pubblicazioni consigliate
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.