Peripheral blood mononuclear cells (PBMCs) were collected from a patient with treatment-refractory Schizophrenia who presented an exceptional clinical response to Clozapine. iPSC lines were established with a non-integrating reprogramming system based on Sendai virus. A footprint-free hiPSC line was characterized to confirm the expression of the main endogenous pluripotency markers and have a regular karyotype. Pluripotency was confirmed by differentiation into cells belonging to the three germ layers. This hiPSC line represents a valuable tool to study the molecular, biochemical and electrophysiological properties of mature neuronal populations belonging to Clozapine responder patients with a severe form of Schizophrenia.

Establishment of an induced pluripotent stem cell (iPSC) line from a patient with Clozapine-responder Schizophrenia / M. Marcatili, F. Marsoner, A. D’Agostino, T. Karnavas, D. Bottai, S. Scarone, L. Conti. - In: STEM CELL RESEARCH. - ISSN 1873-5061. - 17:3(2016), pp. 630-633. [10.1016/j.scr.2016.11.009]

Establishment of an induced pluripotent stem cell (iPSC) line from a patient with Clozapine-responder Schizophrenia

M. Marcatili
Primo
;
A. D’Agostino;D. Bottai;S. Scarone
Penultimo
;
2016

Abstract

Peripheral blood mononuclear cells (PBMCs) were collected from a patient with treatment-refractory Schizophrenia who presented an exceptional clinical response to Clozapine. iPSC lines were established with a non-integrating reprogramming system based on Sendai virus. A footprint-free hiPSC line was characterized to confirm the expression of the main endogenous pluripotency markers and have a regular karyotype. Pluripotency was confirmed by differentiation into cells belonging to the three germ layers. This hiPSC line represents a valuable tool to study the molecular, biochemical and electrophysiological properties of mature neuronal populations belonging to Clozapine responder patients with a severe form of Schizophrenia.
Settore BIO/09 - Fisiologia
Settore BIO/13 - Biologia Applicata
Settore BIO/11 - Biologia Molecolare
Settore BIO/14 - Farmacologia
Settore MED/25 - Psichiatria
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/451092
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