A selective oxygen biosensor based on bilirubin oxidase (BOx) was developed. The sensor was used for determining oxygen profiles in a membraneless, single-chamber microbial fuel cell (SCMFC), fed with raw wastewater. The linear response of the sensor was optimized by a diffusion layer of silica gel. A computer-controlled stage was used to obtain accurate and precise measurements. Oxygen concentration in biofilms covering electrodes was measured, showing 3mgL-1 of O2 in the bulk solution, decreasing to 0mgL-1 in the cathodic biofilm. The MFC generated power in the range of 0-0.08mW, associated to the oxygen content.

Enzymatic Oxygen Microsensor Based on Bilirubin Oxidase Applied to Microbial Fuel Cells Analysis / M. Grattieri, S. Babanova, C. Santoro, E. Guerrini, S.P. Trasatti, P. Cristiani, M. Bestetti, P. Atanassov. - In: ELECTROANALYSIS. - ISSN 1040-0397. - 27:2(2015 Feb), pp. 327-335. [10.1002/elan.201400543]

Enzymatic Oxygen Microsensor Based on Bilirubin Oxidase Applied to Microbial Fuel Cells Analysis

M. Grattieri;E. Guerrini;S.P. Trasatti;
2015-02

Abstract

A selective oxygen biosensor based on bilirubin oxidase (BOx) was developed. The sensor was used for determining oxygen profiles in a membraneless, single-chamber microbial fuel cell (SCMFC), fed with raw wastewater. The linear response of the sensor was optimized by a diffusion layer of silica gel. A computer-controlled stage was used to obtain accurate and precise measurements. Oxygen concentration in biofilms covering electrodes was measured, showing 3mgL-1 of O2 in the bulk solution, decreasing to 0mgL-1 in the cathodic biofilm. The MFC generated power in the range of 0-0.08mW, associated to the oxygen content.
Bilirubin oxidase; Biocathode; Biofilm; Biosensor; MFC; Oxygen measurement; Analytical Chemistry; Electrochemistry
Settore ING-IND/23 - Chimica Fisica Applicata
ELECTROANALYSIS
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/450559
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