A selective oxygen biosensor based on bilirubin oxidase (BOx) was developed. The sensor was used for determining oxygen profiles in a membraneless, single-chamber microbial fuel cell (SCMFC), fed with raw wastewater. The linear response of the sensor was optimized by a diffusion layer of silica gel. A computer-controlled stage was used to obtain accurate and precise measurements. Oxygen concentration in biofilms covering electrodes was measured, showing 3mgL-1 of O2 in the bulk solution, decreasing to 0mgL-1 in the cathodic biofilm. The MFC generated power in the range of 0-0.08mW, associated to the oxygen content.
|Titolo:||Enzymatic Oxygen Microsensor Based on Bilirubin Oxidase Applied to Microbial Fuel Cells Analysis|
GRATTIERI, MATTEO (Primo)
|Parole Chiave:||Bilirubin oxidase; Biocathode; Biofilm; Biosensor; MFC; Oxygen measurement; Analytical Chemistry; Electrochemistry|
|Settore Scientifico Disciplinare:||Settore ING-IND/23 - Chimica Fisica Applicata|
|Data di pubblicazione:||feb-2015|
|Digital Object Identifier (DOI):||10.1002/elan.201400543|
|Appare nelle tipologie:||01 - Articolo su periodico|